Haroen Ucop, Syafwan Syafwan, Kurniawan Kiki, Budiansyah Agus, Widjaja Nilawati, Fakhri Saitul
Faculty of Animal Husbandry, Jambi University, Jambi, Indonesia.
Reseach Center for Vaccine and Drugs Development, National Research and Innovation Agency, Bogor, Indonesia.
J Adv Vet Anim Res. 2025 Mar 25;12(1):192-204. doi: 10.5455/javar.2025.l886. eCollection 2025 Mar.
This study aimed to identify the impact of variations in solvent polarity on the solubility of secondary metabolite compounds, which were correlated as antioxidant and antibacterial agents for (ATCC 11725), (ATCC 22504), (ATCC 11526), and (ATCC 11626).
A total of 500 gm of Temulawak flour () was macerated gradually using the step gradient polarity (SGP) technique, where the solvents (methanol, ethyl acetate, and n-hexane) used had different levels of polarity. The extracting process of secondary metabolite components of Temulawak flour began with the use of non-polar solvents, semipolar solvents, and polar solvents, respectively. Each soaking process was completed for 5 × 24 h.
From the Temulawak extract with different levels of solvent polarity, 76.048 gm were obtained for the methanol fraction, 106.242 gm for the ethyl acetate fraction, and 154.575 gm for the n-hexane fraction. The results of the antioxidant activity test showed that the ethyl acetate fraction had the highest inhibitory value for antioxidant activity of 50% (IC50) 22.59 mg/l. The results of this research's -carotene content were 0.0865 gm. Ethyl acetate fraction was known to have good activity from the antibacterial activity test. The inhibition zone of and bacteria was 11.5 ± 0.71 with a minimum concentration of 3.13 mg/ml. The inhibition zone of was 10.5 ± 0.17 with a minimum inhibitory concentration (MIC) of 6.25 mg/ml, while the inhibition zone for sp. was 8.125 ± 0.35 with a MIC of 37.50 mg/ml.
All fractions have moderate antibacterial activity, yet the ability of the ethyl acetate fraction of Temulawak extract was higher than the methanol and n-hexane fractions.
本研究旨在确定溶剂极性变化对次生代谢物化合物溶解度的影响，这些次生代谢物化合物与作为抗氧化剂和抗菌剂的（美国典型培养物保藏中心11725）、（美国典型培养物保藏中心22504）、（美国典型培养物保藏中心11526）和（美国典型培养物保藏中心11626）相关。
使用梯度极性（SGP）技术逐步浸渍总共500克的高良姜粉（），所使用的溶剂（甲醇、乙酸乙酯和正己烷）具有不同程度的极性。高良姜粉次生代谢物成分的提取过程分别从不极性溶剂、半极性溶剂和极性溶剂开始。每个浸泡过程均持续5×24小时。
从具有不同溶剂极性水平的高良姜提取物中，甲醇馏分得到76.048克，乙酸乙酯馏分得到106.242克，正己烷馏分得到154.575克。抗氧化活性测试结果表明，乙酸乙酯馏分对抗氧化活性的抑制值最高，50%（IC50）为22.59毫克/升。本研究的β-胡萝卜素含量结果为0.0865克。从抗菌活性测试可知乙酸乙酯馏分具有良好活性。金黄色葡萄球菌和大肠杆菌的抑菌圈为11.5±0.71，最低浓度为3.13毫克/毫升。铜绿假单胞菌的抑菌圈为10.5±0.17，最低抑菌浓度（MIC）为6.25毫克/毫升，而对白色念珠菌的抑菌圈为8.125±0.35，MIC为37.50毫克/毫升。
所有馏分均具有中等抗菌活性，但高良姜提取物的乙酸乙酯馏分的能力高于甲醇和正己烷馏分。
