School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan, 030006, China.
Food Colloids Group, School of Food Science and Nutrition, University of Leeds, Leeds, LS2 9JT, UK.
Angew Chem Int Ed Engl. 2022 Nov 7;61(45):e202211912. doi: 10.1002/anie.202211912. Epub 2022 Oct 12.
Co-immobilization of enzymes and cofactors in a manner suitable for use in continuous flow catalysis remains a great challenge because of the difficulty in ensuring the free accessibility of immobilized enzymes and cofactors. Herein, we present a continuous flow catalysis system based on co-compartmentalization of enzymes and cofactors within Pickering emulsion droplets, enabling regeneration of cofactors within the droplets. As exemplified by enzyme-catalyzed ketone enantioselective reduction and enantioselective transamination, our systems exhibit long-term stability (300-400 h), outstanding total turnover number (TTN, 59204 mol mol ) and several-fold enhancement in the enzyme catalytic efficiency (CE ) in comparison to conventional biphasic reactions. As well as giving insight into the co-compartmentalization effects, our system will provide the opportunity to significantly advance continuous-flow biocatalysis towards the level of practical applications.
由于难以确保固定化酶和辅因子的自由可及性，将酶和辅因子以适合连续流催化的方式共固定仍然是一个巨大的挑战。在此，我们提出了一种基于酶和辅因子在 Pickering 乳液液滴内共分隔的连续流催化体系，从而实现了液滴内辅因子的再生。以酶催化酮对映选择性还原和对映选择性转氨为例，与传统两相反应相比，我们的体系表现出长期稳定性（300-400 h）、出色的总转化数（TTN，59204 mol mol ）和数倍提高的酶催化效率（CE）。除了深入了解共分隔效应外，我们的体系还将为连续流生物催化向实际应用水平的显著推进提供机会。
