Zygmuntowicz Aleksandra, Markiewicz Włodzimierz, Grabowski Tomasz, Jaroszewski Jerzy
Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, 10-718 Olsztyn, Poland.
Department of Inorganic Chemistry, Faculty of Pharmacy, Medical University of Gdańsk, 80-416 Gdańsk, Poland.
J Vet Res. 2022 Jul 5;66(2):257-265. doi: 10.2478/jvetres-2022-0031. eCollection 2022 Jun.
Bisphenols, as endocrine disruptors, may cause a wide range of health problems in humans, but so far, not all of them have been confirmed in animals, including pigs. Since animals are also exposed to bisphenols, we hypothesised that these substances may have an effect on uterine contractility in pigs. Therefore, the aim of the study was to investigate the effect of the most-used bisphenol, bisphenol A (BPA), and a selected analogue, bisphenol F (BPF), on the contractile activity of the pig uterus.
The investigation utilised smooth muscles from immature pigs (n = 6), cyclic pigs on days 12-14 of the oestrous cycle (n = 6) or early pregnant pigs on days 12-16 of pregnancy (n = 6). Strips of the myometrium were exposed to BPA and BPF at concentrations of 10-10 M. Smooth muscle contractility was determined with equipment for measuring isometric contractions.
BPA caused a significant decrease in contraction amplitude, and frequency and in myometrial tension in all groups examined. BPF caused a decrease in the amplitude and frequency of contractions in all groups and in myometrial tension in the early pregnant group.
The obtained results indicate that both BPA and BPF relaxed the porcine myometrium, but these changes, especially in the amplitude and frequency of contractions, were more evident after BPF treatment. The extent of relaxation is dependent on the physiological status of the animals.
双酚类物质作为内分泌干扰物，可能会给人类带来一系列健康问题，但迄今为止，并非所有问题都在包括猪在内的动物身上得到证实。由于动物也会接触双酚类物质，我们推测这些物质可能会对猪的子宫收缩产生影响。因此，本研究的目的是调查最常用的双酚——双酚A（BPA）和一种选定的类似物双酚F（BPF）对猪子宫收缩活性的影响。
本研究使用了未成熟猪（n = 6）、发情周期第12 - 14天的周期性猪（n = 6）或怀孕第12 - 16天的早期妊娠猪（n = 6）的平滑肌。将子宫肌层条带暴露于浓度为10 - 10 M的BPA和BPF中。使用测量等长收缩的设备测定平滑肌收缩性。
BPA导致所有受试组的收缩幅度、频率和子宫肌层张力显著降低。BPF导致所有组的收缩幅度和频率降低，早期妊娠组的子宫肌层张力降低。
所得结果表明，BPA和BPF均可使猪子宫肌层松弛，但这些变化，尤其是收缩幅度和频率方面的变化，在BPF处理后更为明显。松弛程度取决于动物的生理状态。
