Prechl József, Papp Krisztián, Kovács Ágnes, Pfeil Tamás
R&D Laboratory, Diagnosticum Zrt, 1047 Budapest, Hungary.
Department of Applied Analysis and Computational Mathematics, Eötvös Loránd University, 1117 Budapest, Hungary.
Antibodies (Basel). 2022 Jun 23;11(3):43. doi: 10.3390/antib11030043.
Antibodies constitute a major component of serum on protein mass basis. We also know that the structural diversity of these antibodies exceeds that of all other proteins in the body and they react with an immense number of molecular targets. What we still cannot quantitatively describe is how antibody abundance is related to affinity, specificity, and cross reactivity. This ignorance has important practical consequences: we also do not have proper biochemical units for characterizing polyclonal serum antibody binding. The solution requires both a theoretical foundation, a physical model of the system, and technology for the experimental confirmation of theory. Here we argue that the quantitative characterization of interactions between serum antibodies and their targets requires systems-level physical chemistry approach and generates results that should help create maps of antibody binding landscape.
基于蛋白质质量，抗体是血清的主要组成部分。我们也知道，这些抗体的结构多样性超过了体内所有其他蛋白质，并且它们能与大量分子靶点发生反应。我们仍然无法定量描述的是抗体丰度与亲和力、特异性和交叉反应性之间的关系。这种无知具有重要的实际后果：我们也没有合适的生化单位来表征多克隆血清抗体结合。解决方案既需要一个理论基础、该系统的物理模型，也需要用于理论实验验证的技术。在此我们认为，血清抗体与其靶点之间相互作用的定量表征需要系统层面的物理化学方法，并能产生有助于绘制抗体结合图谱的结果。
