绿藻来源硫酸葡聚糖通过抑制脂肪生成和脂质生成关键因子来抑制脂肪生成。
Sulfated Glucan from the Green Seaweed Inhibits Adipogenesis through Suppression of Adipogenic and Lipogenic Key Factors.
机构信息
Laboratory of Molecular and Genomic Biology, Department of Biology and Genetics, Center of Biosciences, Federal University of Rio Grande do Norte, Natal 59072-900, RN, Brazil.
The Doctoral Program in Biotechnology-Northeast Biotechnology Network (RENORBIO), Center of Biosciences, Federal University of Rio Grande do Norte, Natal 59072-900, RN, Brazil.
出版信息
Mar Drugs. 2022 Jul 23;20(8):470. doi: 10.3390/md20080470.
Sulfated polysaccharides (SPS) from seaweeds have great biochemical and biotechnological potential. This study aimed to investigate the effect of SPS isolated from the seaweed on adipogenic differentiation as a possible alternative treatment for obesity. The SPS-rich extract from the seaweed was fractioned into three SPS-rich fractions (F0.5; F0.9; and F1.8) chemically characterized. Among these four samples, only F0.9 showed a significant inhibitory effect on adipogenesis of 3T3-L1 preadipocytes. Ten SPS-rich fractions were isolated from F0.9 through ion-exchange chromatography. However, only the fraction (CS0.2) containing a sulfated glucan was able to inhibit adipogenesis. CS0.2 reduces lipid accumulation and inhibits the expression of key adipogenic (PPARγ, C/EBPβ, and C/EBPα) and lipogenic markers (SREBP-1c, Fabp4, and CD36). The data points to the potential of sulfated glucan from for the development of functional approaches in obesity management.
海藻中的硫酸多糖（SPS）具有巨大的生化和生物技术潜力。本研究旨在探讨从海藻中分离出的 SPS 对脂肪生成分化的影响，以期为肥胖的治疗提供一种新方法。对海藻 SPS 丰富的提取物进行分级分离，得到三个 SPS 丰富的级分（F0.5；F0.9；F1.8），并对其进行化学表征。在这四个样品中，只有 F0.9 对 3T3-L1 前脂肪细胞的脂肪生成具有显著的抑制作用。从 F0.9 中通过离子交换色谱分离出 10 个 SPS 丰富的级分。然而，只有含有硫酸葡聚糖的级分（CS0.2）能够抑制脂肪生成。CS0.2 减少了脂质积累，并抑制了关键的脂肪生成（PPARγ、C/EBPβ 和 C/EBPα）和脂肪生成标志物（SREBP-1c、Fabp4 和 CD36）的表达。这些数据表明，来自 的硫酸葡聚糖具有开发肥胖症管理功能性方法的潜力。
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