Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Agronomic Sciences and Veterinary Medicine of Bucharest, 105 Splaiul Independentei, District 5, 050097 Bucharest, Romania.
Laboratory of Molecular Plant Physiology, Research Center for Studies of Food Quality and Agricultural Products, University of Agronomic Sciences and Veterinary Medicine of Bucharest, 59 Marasti Blvd, District 1, 011464 Bucharest, Romania.
Genes (Basel). 2022 Jul 23;13(8):1316. doi: 10.3390/genes13081316.
The variability of prion protein gene () codons and the frequency of alleles (K222, D146, and S146) that appear to confer genetic resistance to classical scrapie are still unknown in several goat populations/breeds prevalent in Romania. This work aims to assess the genetic polymorphism at 15 codons in Romanian goat populations to inform the development of goat breeding programs for scrapie resistance.
Whole blood and hair follicles from Carpathian (50), French Alpine (53), and Banat's White (53) breed goats were sampled to extract genomic DNA for genetic analyses and Sanger sequencing. In the targeted goat groups, one classical scrapie-positive Banat's White goat was included.
The codons without polymorphisms were G37G, W102W, N146N, R151R, S173S, and I218I. The following non-synonymous polymorphisms of were recorded: P110P, P110S, P110T, T110T, G127G, G127S, I142I, I142M, T142I, H143H, P143P, R143R, R154R, H154R, P168P, Q168Q, Q211Q, Q211R, Q222Q, H222Q, K222K, S240S, P240P, P240S, and S240P.
polymorphism was recorded in 60% (9/15) of codons. The scrapie-positive Banat's White goat had G37G, W102W, T110T, G127G, I142I, H143H, N146N, R151R, R154R, P168P, S173S, R211R, I218I, Q222Q, and S240S. The K222 allele had a frequency of 6% (3/50) in Carpathian, 9.43% (5/53) in Banat's White, and 15.09% (8/53) in French Alpine. Therefore, the polymorphisms detected in this sample of Romanian goat breeds are too rare to design a breeding program at the current time.
在罗马尼亚流行的几种山羊群体/品种中，朊病毒蛋白基因（）密码子的变异性和似乎赋予对经典羊瘙痒病遗传抗性的等位基因（K222、D146 和 S146）的频率尚不清楚。这项工作旨在评估罗马尼亚山羊群体中 15 个密码子的遗传多态性，为抗瘙痒病的山羊育种计划提供信息。
从喀尔巴阡山脉（50 只）、法国阿尔卑斯山（53 只）和巴纳特白山羊（53 只）中采集血样和毛囊，以提取基因组 DNA 进行遗传分析和 Sanger 测序。在目标山羊群体中，包括一只经典羊瘙痒病阳性的巴纳特白山羊。
无多态性的密码子为 G37G、W102W、N146N、R151R、S173S 和 I218I。记录了以下非同义的 多态性：P110P、P110S、P110T、T110T、G127G、G127S、I142I、I142M、T142I、H143H、P143P、R143R、R154R、H154R、P168P、Q168Q、Q211Q、Q211R、Q222Q、H222Q、K222K、S240S、P240P、P240S 和 S240P。
60%（9/15）的密码子记录了 多态性。瘙痒病阳性的巴纳特白山羊具有 G37G、W102W、T110T、G127G、I142I、H143H、N146N、R151R、R154R、P168P、S173S、R211R、I218I、Q222Q 和 S240S。K222 等位基因在喀尔巴阡山脉中的频率为 6%（3/50），在巴纳特白山羊中的频率为 9.43%（5/53），在法国阿尔卑斯山的频率为 15.09%（8/53）。因此，目前在这个罗马尼亚山羊品种样本中检测到的多态性太罕见，无法设计一个育种计划。
