Wang Jianhua, Lin Shanhai, Zhu Guanghui, Zhao Zhiyong, Wang Haoyu, Zhou Miaoping, Zhao Xingming, Wu Aibo
Institute for Agri-Food Standards and Testing Technology, Shanghai Academy of Agricultural Sciences, 1000 Jinqi Road, Shanghai 201403, China.
Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China.
Microorganisms. 2022 Jul 22;10(8):1479. doi: 10.3390/microorganisms10081479.
The species complex is the main causal agent of wheat head blight worldwide. Trichothecenes produced by the pathogen in infected grains have important food safety implications. Previously reported studies on trichothecene production have all focused on the conditions conducive to mycotoxin production, while the molecular mechanisms of trichothecene biosynthesis in strains under normal or non-inducing conditions are still unclear. Here, a global analysis of the fungal gene expression of three strains using the Affymetrix GeneChip under non-inducing conditions is reported. Differentially expressed genes were identified among strains with different trichothecene-production ability, and some novel genes associated with trichothecene biosynthesis were found by bioinformatics analysis. To verify the transcriptome results, proteomic analyses of the three strains were conducted under the same culture conditions. In total, 69 unique fungal proteins were identified in 77 protein spots. Combined with transcriptome and proteome analysis, 27 novel genes were predicted to be associated with trichothecene mycotoxin production. A protein, encoded by , was found to be associated with trichothecene production via proteome analysis. Gene knock-out mutations of resulted in mutants with increased production of trichothecenes. Future functional analysis of the candidate genes identified in this study may reveal new insights into the negative regulation of trichothecene production in the species complex.
该物种复合体是全球小麦赤霉病的主要致病因子。病原体在受感染谷物中产生的单端孢霉烯族毒素对食品安全具有重要影响。先前关于单端孢霉烯族毒素产生的研究都集中在有利于霉菌毒素产生的条件上，而在正常或非诱导条件下菌株中单端孢霉烯族毒素生物合成的分子机制仍不清楚。本文报道了在非诱导条件下使用Affymetrix基因芯片对三株菌株的真菌基因表达进行的全面分析。在具有不同单端孢霉烯族毒素产生能力的菌株中鉴定出差异表达基因，并通过生物信息学分析发现了一些与单端孢霉烯族毒素生物合成相关的新基因。为了验证转录组结果，在相同培养条件下对这三株菌株进行了蛋白质组分析。总共在77个蛋白斑点中鉴定出69种独特的真菌蛋白。结合转录组和蛋白质组分析，预测有27个新基因与单端孢霉烯族霉菌毒素的产生有关。通过蛋白质组分析发现一种由……编码的蛋白质与单端孢霉烯族毒素的产生有关。……的基因敲除突变导致突变体中单端孢霉烯族毒素产量增加。本研究中鉴定出的候选基因的未来功能分析可能会揭示该物种复合体中单端孢霉烯族毒素产生负调控的新见解。
