Juliawan I Made Putra, Suwana Febie Putra, Annas Jimmy Yanuar, Akbar Muhammad Firman, Widjiati Widjiati
Department of Obstetrics and Gynecology, Faculty of Medicine, Airlangga University, Surabaya 75320, Indonesia.
Department of Obstetrics and Gynecology, Faculty of Medicine, The University of Mataram, Mataram 83115, Indonesia.
Pathophysiology. 2022 Jul 8;29(3):344-353. doi: 10.3390/pathophysiology29030026.
Polycystic ovary syndrome (PCOS), a common hormonal disorder in women of reproductive age, is associated with a poor and unhealthy diet. This study aimed to investigate the effect of a high sucrose and cholic acid (HSCA) diet in the presence of PCOS-like phenotypes. Female Wistar rats were divided into HSCA and normal diet groups for four weeks, each with twenty rats. Body weight was assessed before and after the study. Blood and fecal samples were obtained to measure HOMA-IR and testosterone level (ELISA) and Enterobacteriaceae isolates grown on MacConkey Agar. Obtained ovarian tissues were H&E-stained. HSCA rats demonstrated a reduction in Enterobacteriaceae colonies (median 4.75 × 105 vs. 2.47 × 104/CFU, p < 0.001) and an elevated HOMA-IR (mean 2.94 ± 1.30 vs. 4.92 ± 0.51, p < 0.001), as well as an increase in testosterone level (median 0.65 vs. 3.00 ng/mL, p < 0.001), despite no statistical differences in the change in body weight (mean −2.31 ± 14.42 vs. −3.45 ± 9.32, p = 0.769). In H&E staining, HSCA rats had a reduction in preovulatory follicle count (median 0.50 vs. 0.00, p = 0.005). The HSCA diet caused insulin resistance and high testosterone levels, which contribute to the development of PCOS, and affected folliculogenesis by altering follicular maturation, but had no effect on ovulation.
多囊卵巢综合征（PCOS）是育龄期女性常见的激素紊乱疾病，与不良和不健康饮食有关。本研究旨在探讨高蔗糖和胆酸（HSCA）饮食在多囊卵巢综合征样表型存在时的影响。将雌性Wistar大鼠分为HSCA组和正常饮食组，为期四周，每组20只大鼠。在研究前后评估体重。采集血液和粪便样本以测量胰岛素抵抗指数（HOMA-IR）和睾酮水平（酶联免疫吸附测定法），并检测在麦康凯琼脂上生长的肠杆菌科分离菌。对获取的卵巢组织进行苏木精-伊红（H&E）染色。HSCA组大鼠的肠杆菌科菌落减少（中位数4.75×10⁵ vs. 2.47×10⁴/CFU，p<0.001），胰岛素抵抗指数升高（平均值2.94±1.30 vs. 4.92±0.51，p<0.001），睾酮水平也升高（中位数0.65 vs. 3.00 ng/mL，p<0.001），尽管体重变化无统计学差异（平均值-2.31±14.42 vs. -3.45±9.32，p = 0.769）。在H&E染色中，HSCA组大鼠排卵前卵泡数量减少（中位数0.50 vs. 0.00，p = 0.005）。HSCA饮食导致胰岛素抵抗和高睾酮水平，这有助于多囊卵巢综合征的发展，并通过改变卵泡成熟影响卵泡发生，但对排卵无影响。
