National Food Institute, Technical University of Denmark, Kgs Lyngby, Denmark.
Department of Hepatology and Gastroenterology, Aarhus University Hospital, Aarhus, Denmark.
Appl Environ Microbiol. 2021 May 11;87(11). doi: 10.1128/AEM.00395-21.
Assessing "dysbiosis" in intestinal microbial communities is increasingly considered a routine analysis in microbiota studies, and it has added relevant information to the prediction and characterization of diseases and other adverse conditions. However, dysbiosis is not a well-defined condition. A variety of different dysbiosis indexes have been suggested and applied, but their underlying methodologies, as well as the cohorts and conditions for which they have been developed, differ considerably. To date, no comprehensive overview and comparison of all the different methodologies and applications of such indexes is available. Here, we list all types of dysbiosis indexes identified in the literature, introduce their methodology, group them into categories, and discuss their potential descriptive and clinical applications as well as their limitations. Thus, our focus is not on the implications of dysbiosis for disease but on the methodological approaches available to determine and quantify this condition.
评估肠道微生物群落中的“生态失调”，在微生物组研究中越来越被视为一种常规分析，它为疾病和其他不良状况的预测和特征描述提供了相关信息。然而，生态失调并不是一种明确定义的状态。已经提出并应用了多种不同的生态失调指标，但它们的基础方法以及开发它们所针对的队列和条件有很大的不同。迄今为止，尚无关于这些指标的所有不同方法和应用的全面概述和比较。在这里，我们列出了文献中确定的所有类型的生态失调指标，介绍了它们的方法，将它们分为几类，并讨论了它们作为描述性和临床应用的潜力以及它们的局限性。因此，我们的重点不是生态失调对疾病的影响，而是可用于确定和量化这种情况的方法方法。
