Kang Lihua, Teng Yangyang, Cen Qiwen, Fang Yunxia, Tian Quanxiang, Zhang Xiaoqin, Wang Hua, Zhang Xian, Xue Dawei
College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China.
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
Plants (Basel). 2022 Jul 25;11(15):1928. doi: 10.3390/plants11151928.
The myeloblastosis (MYB) family comprises a large group of transcription factors (TFs) that has a variety of functions. Among them, the R2R3-MYB type of proteins are the largest group in plants, which are involved in controlling various biological processes such as plant growth and development, physiological metabolism, defense, and responses to abiotic and biotic stresses. In this study, bioinformatics was adopted to conduct genome-wide identification of the R2R3-MYB TFs in rice. We identified 190 MYB TFs (99 R2R3-MYBs), which are unevenly distributed on the 12 chromosomes of rice. Based on the phylogenetic clustering and protein sequence characteristics, OsMYBs were classified into five subgroups, and 59.6% of the genes contained two introns. Analysis of cis-acting elements in the 2000 bp upstream region of genes showed that all genes contained plant hormones-related or stress-responsive elements since 91.9%, 79.8%, 79.8%, and 58.6% of genes contain ABRE, TGACG, CGTCA, and MBS motifs, respectively. Protein-protein network analysis showed that the were involved in metabolic process, biosynthetic process, and tissue development. In addition, some genes showed a tissue-specific or developmental-stage-specific expression pattern. Moreover, the transcription levels of 20 genes under polyethylene glycol (PEG) and cadmium chloride (CdCl) stress inducers were dissected by qRT-PCR. The results indicated genes with an altered expression upon PEG or CdCl stress induction. These results potentially supply a basis for further research on the role that genes play in plant development and stress responses.
成髓细胞瘤（MYB）家族由一大类具有多种功能的转录因子（TFs）组成。其中，R2R3-MYB类型的蛋白质是植物中最大的一类，参与控制各种生物学过程，如植物生长发育、生理代谢、防御以及对非生物和生物胁迫的响应。在本研究中，采用生物信息学方法对水稻中的R2R3-MYB转录因子进行全基因组鉴定。我们鉴定出190个MYB转录因子（99个R2R3-MYB），它们在水稻的12条染色体上分布不均。基于系统发育聚类和蛋白质序列特征，将OsMYBs分为五个亚组，59.6%的基因含有两个内含子。对基因上游2000 bp区域的顺式作用元件分析表明，所有基因都含有与植物激素相关或胁迫响应元件，因为分别有91.9%、79.8%、79.8%和58.6%的基因含有ABRE、TGACG、CGTCA和MBS基序。蛋白质-蛋白质网络分析表明，这些基因参与代谢过程、生物合成过程和组织发育。此外，一些基因表现出组织特异性或发育阶段特异性的表达模式。此外，通过qRT-PCR分析了20个基因在聚乙二醇（PEG）和氯化镉（CdCl）胁迫诱导下的转录水平。结果表明，基因在PEG或CdCl胁迫诱导下表达发生改变。这些结果可能为进一步研究这些基因在植物发育和胁迫响应中的作用提供基础。
