Chung Yao-Chi, Cheng Li-Ting, Zhang Jia-Yu, Wu Yue-Jyun, Liu Shyh-Shyan, Chu Chun-Yen
Graduate Institute of Animal Vaccine Technology, College of Veterinary Medicine, National Pingtung University of Science and Technology, 1, Shuehfu Road, Neipu, Pingtung, 91201, Taiwan.
Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, 1, Shuehfu Road, Neipu, Pingtung, 91201, Taiwan.
BMC Vet Res. 2018 Jun 19;14(1):194. doi: 10.1186/s12917-018-1520-2.
Inactivated and subunit bovine viral diarrhea virus (BVDV) vaccines have shown limited protective efficacy. This study aimed to evaluate the effectiveness of a vaccine containing both inactivated BVDV (iBVDV) and baculovirus-expressed recombinant E2 (rE2), an important BVDV antigen with strongly neutralizing epitopes.
Four groups of goats were immunized twice with one of four vaccine preparations: 1) iBVDV+rE2, 2) rE2, 3) iBVDV, and 4) saline, and challenged with BVDV. For goats vaccinated with the iBVDV+rE2 vaccine, no viremia was observed after challenge, and clinical signs, pyrexia, and leukopenia were reduced compared to the saline group. In contrast, for goats vaccinated with either iBVDV or rE2 alone, viremia was still detectable.
The combination of iBVDV and rE2 elicited stronger protective immune responses against BVDV than iBVDV or rE2 alone.
灭活和亚单位牛病毒性腹泻病毒（BVDV）疫苗的保护效果有限。本研究旨在评估一种包含灭活BVDV（iBVDV）和杆状病毒表达的重组E2（rE2）的疫苗的有效性，rE2是一种具有强中和表位的重要BVDV抗原。
四组山羊用四种疫苗制剂之一进行两次免疫：1）iBVDV+rE2，2）rE2，3）iBVDV，4）生理盐水，然后用BVDV进行攻毒。对于接种iBVDV+rE2疫苗的山羊，攻毒后未观察到病毒血症，与生理盐水组相比，临床症状、发热和白细胞减少症有所减轻。相比之下，对于单独接种iBVDV或rE2的山羊，仍可检测到病毒血症。
iBVDV和rE2联合使用比单独使用iBVDV或rE2能引发更强的针对BVDV的保护性免疫反应。
