Dietzel I, Kolb V, Boos W
Arch Microbiol. 1978 Aug 1;118(2):207-18. doi: 10.1007/BF00415731.
After induction with maltose, 30--40% of the total protein in the osmotic shock fluid consist of maltose-binding protein while the induction ratio (maltose versus glycerol grown cells) for the amount of binding protein synthesized as well as for maltose transport is in the order of 10. Induction of maltose transport does not occur during all times of the cell cycle, but only shortly before cell division. Electronmicroscopic analysis of cells grown logarithmically on glycerol or maltose revealed in the latter the formation of large pole caps. These pole caps arise from an enlargement of the periplasmic space. Small cells contain one pole cap, large cells contain two. Pulse label studies with strain BUG-6, a mutant that is temperature sensitive for cell division reveal the following: Growth at the non-permissive temperature prevents maltose-binding protein synthesis and formation of new transport capacity. After shifting to the permissive temperature the cells regain both functions. Simultaneously, the newly formed cells exhibit pole caps. We conclude that the induction of maltose-binding protein is responsible for the formation of pole caps. In addition, beside the presence of inducer, cell cycle events occuring during division are necessary for the synthesis of maltose-binding protein.
用麦芽糖诱导后，渗透休克液中总蛋白的30% - 40%由麦芽糖结合蛋白组成，而合成的结合蛋白量以及麦芽糖转运的诱导率（麦芽糖与甘油培养的细胞相比）约为10。麦芽糖转运的诱导并非在细胞周期的所有时间都发生，而是仅在细胞分裂前不久发生。对在甘油或麦芽糖上对数生长的细胞进行电子显微镜分析发现，在后者中形成了大的极帽。这些极帽源于周质空间的扩大。小细胞含有一个极帽，大细胞含有两个。对BUG - 6菌株（一种对细胞分裂温度敏感的突变体）进行脉冲标记研究，结果如下：在非允许温度下生长会阻止麦芽糖结合蛋白的合成和新转运能力的形成。转移到允许温度后，细胞恢复这两种功能。同时，新形成的细胞呈现出极帽。我们得出结论，麦芽糖结合蛋白的诱导负责极帽的形成。此外，除了诱导剂的存在外，分裂过程中发生的细胞周期事件对于麦芽糖结合蛋白的合成也是必要的。
