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大肠杆菌K-12中的麦芽糖转运:噬菌体λ受体的作用。

Maltose transport in Escherichia coli K-12: involvement of the bacteriophage lambda receptor.

作者信息

Szmelcman S, Hofnung M

出版信息

J Bacteriol. 1975 Oct;124(1):112-8. doi: 10.1128/jb.124.1.112-118.1975.

Abstract

Mutants affected in lamB, the structural gene for phage lambda receptor, are unable to utilize maltose when it is present at low concentrations (less than or equal 10 muM). During growth in a chemostat at limiting maltose concentrations, the lamB mutants tested were selected against in the presence of the wild-type strain. Transport studies demonstrate that most lamB mutants have deficient maltose transport capacities at low maltose concentrations. When antibodies against purified phage lambda receptor are added to a wild-type strain, transport of maltose at low concentrations is significantly reduced. These results strongly suggest that the phage lambda receptor molecule is involved in maltose transport.

摘要

在噬菌体λ受体的结构基因lamB中发生突变的突变体,当麦芽糖以低浓度(小于或等于10μM)存在时无法利用它。在恒化器中于限制麦芽糖浓度下生长时,所测试的lamB突变体在野生型菌株存在的情况下被淘汰。转运研究表明,大多数lamB突变体在低麦芽糖浓度下具有缺陷的麦芽糖转运能力。当将针对纯化的噬菌体λ受体的抗体添加到野生型菌株中时,低浓度麦芽糖的转运显著降低。这些结果强烈表明,噬菌体λ受体分子参与麦芽糖转运。

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