Azizi Machekposhti Sina, Nguyen Alexander K, Vanderwal Lyndsi, Stafslien Shane, Narayan Roger J
Joint UNC/NCSU Department of Biomedical Engineering, North Carolina State University, Raleigh, NC 27695, USA.
Coatings and Polymeric Materials, North Dakota State University, Fargo, ND 58102, USA.
Pharmaceutics. 2022 Jul 26;14(8):1551. doi: 10.3390/pharmaceutics14081551.
Biocompatible and biodegradable materials have been used for fabricating polymeric microneedles to deliver therapeutic drug molecules through the skin. Microneedles have advantages over other drug delivery methods, such as low manufacturing cost, controlled drug release, and the reduction or absence of pain. The study examined the delivery of amphotericin B, an antifungal agent, using microneedles that were fabricated using a micromolding technique. The microneedle matrix was made from Gantrez AN-119 BF, a benzene-free methyl vinyl ether/maleic anhydride copolymer. The Gantrez AN-119 BF was mixed with water; after water evaporation, the polymer exhibited sufficient strength for microneedle fabrication. Molds cured at room temperature remained sharp and straight. SEM images showed straight and sharp needle tips; a confocal microscope was used to determine the height and tip diameter for the microneedles. Nanoindentation was used to obtain the hardness and Young's modulus values of the polymer. Load-displacement testing was used to assess the failure force of the needles under compressive loading. These two mechanical tests confirmed the mechanical properties of the needles. In vitro studies validated the presence of amphotericin B in the needles and the antifungal properties of the needles. Amphotericin B Gantrez microneedles fabricated in this study showed appropriate characteristics for clinical translation in terms of mechanical properties, sharpness, and antifungal properties.
生物相容性和可生物降解的材料已被用于制造聚合物微针，以通过皮肤递送治疗性药物分子。与其他药物递送方法相比，微针具有诸多优势，如制造成本低、药物释放可控以及减轻或消除疼痛等。该研究使用微成型技术制造的微针，研究了抗真菌剂两性霉素B的递送情况。微针基质由Gantrez AN - 119 BF制成，这是一种无苯的甲基乙烯基醚/马来酸酐共聚物。将Gantrez AN - 119 BF与水混合；水蒸发后，聚合物表现出足够的强度用于制造微针。在室温下固化的模具保持尖锐和笔直。扫描电子显微镜图像显示针尖笔直且尖锐；使用共聚焦显微镜确定微针的高度和针尖直径。采用纳米压痕法获得聚合物的硬度和杨氏模量值。使用载荷 - 位移测试评估针在压缩载荷下的破坏力。这两项力学测试证实了针的力学性能。体外研究验证了微针中两性霉素B的存在以及微针的抗真菌特性。本研究制造的两性霉素B Gantrez微针在力学性能、尖锐度和抗真菌特性方面显示出适合临床转化的特性。
