Joint Department of Biomedical Engineering, University of North Carolina, Raleigh, USA.
Biofabrication. 2012 Mar;4(1):011002. doi: 10.1088/1758-5082/4/1/011002. Epub 2012 Jan 30.
Microneedles are needle-like projections with microscale features that may be used for transdermal delivery of a variety of pharmacologic agents, including antibacterial agents. In the study described in this paper, an indirect rapid prototyping approach involving a combination of visible light dynamic mask micro-stereolithography and micromolding was used to prepare microneedle arrays out of a biodegradable acid anhydride copolymer, Gantrez(®) AN 169 BF. Fourier transform infrared spectroscopy, energy dispersive x-ray spectrometry and nanoindentation studies were performed to evaluate the chemical and mechanical properties of the Gantrez(®) AN 169 BF material. Agar plating studies were used to evaluate the in vitro antimicrobial performance of these arrays against Bacillus subtilis, Candida albicans, Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. Large zones of growth inhibition were noted for Escherichia coli, S. aureus, Enterococcus faecalis and B. subtilis. The performance of Gantrez(®) AN 169 BF against several bacteria suggests that biodegradable acid anhydride copolymer microneedle arrays prepared using visible light dynamic mask micro-stereolithography micromolding may be useful for treating a variety of skin infections.
微针是具有微观特征的针状突起，可用于经皮递送达多种药物制剂，包括抗菌剂。在本文所述的研究中，使用间接快速原型制作方法，将可见光动态掩模微立体光刻和微成型相结合，由可生物降解的酸酐共聚物 Gantrez® AN 169 BF 制备微针阵列。傅里叶变换红外光谱、能量色散 X 射线光谱和纳米压痕研究用于评估 Gantrez® AN 169 BF 材料的化学和机械性能。琼脂平板研究用于评估这些阵列对枯草芽孢杆菌、白色念珠菌、粪肠球菌、大肠杆菌、铜绿假单胞菌和金黄色葡萄球菌的体外抗菌性能。大肠杆菌、金黄色葡萄球菌、粪肠球菌和枯草芽孢杆菌的生长抑制区域较大。Gantrez® AN 169 BF 对几种细菌的作用表明，使用可见光动态掩模微立体光刻微成型制备的可生物降解酸酐共聚物微针阵列可能可用于治疗各种皮肤感染。
