Shukla Shubhangi, Machekposhti Sina Azizi, Joshi Naveen, Joshi Pratik, Narayan Roger J
Joint Department of Biomedical Engineering North Carolina State University Raleigh NC 27695-7907 USA.
Department of Materials Science and Engineering North Carolina State University Raleigh NC 27695-7907 USA.
Small Sci. 2023 Apr 22;3(6):2200087. doi: 10.1002/smsc.202200087. eCollection 2023 Jun.
Currently available point-of-care systems for body fluid collection exhibit poor integration with sensors. Herein, the design of a disposable device for interstitial fluid (ISF) extraction as well as glucose, lactate, and potassium ion (K) monitoring is reported on. It is minimally invasive and appropriate for single use, minimizing the risk of infection to the user. This microscale device contains a 3D-printed cap-like structure with a four-by-four microneedle (MN) array, bioreceptor-modified carbon fiber (CF)-sensing surface, and negative pressure convection technology. These features are incorporated within a compact, self-contained, and manually operated microscale device, which is capable of withdrawing ≈3.0 μL of ISF from the skin. MN arrays applied with an upward driving force may increase the ISF flow rate. Moreover, functionalized CF working electrodes (WE, WE, WE) are shown to selectively detect lactate, glucose, and K with high sensitivities of 0.258, 0.549, and 0.657 μA μm cm and low detection limits of 0.01, 0.080, 0.05 μm, respectively. Ex vivo testing on porcine skin is used to detect the ISF levels of the biomarkers. The microscale device can be a replacement for current point-of-care diagnostic approaches.
目前可用的用于体液采集的即时检测系统与传感器的集成性较差。在此，本文报道了一种用于提取组织间液（ISF）以及监测葡萄糖、乳酸和钾离子（K）的一次性装置的设计。该装置微创且适合一次性使用，将用户感染风险降至最低。这种微型装置包含一个3D打印的帽状结构，带有一个4×4微针（MN）阵列、生物受体修饰的碳纤维（CF）传感表面和负压对流技术。这些特性被整合在一个紧凑、独立且手动操作的微型装置中，该装置能够从皮肤中抽取约3.0μL的ISF。施加向上驱动力的MN阵列可能会提高ISF流速。此外，功能化的CF工作电极（WE、WE、WE）显示出能够分别以0.258、0.549和0.657μAμm cm的高灵敏度以及0.01、0.080、0.05μm的低检测限选择性地检测乳酸、葡萄糖和K。在猪皮肤上进行的体外测试用于检测生物标志物的ISF水平。这种微型装置可以替代当前的即时检测诊断方法。
