Wang Yifang, Zhang Yihua, He Yinting, Zhang Shixun, Huang Bo, Wang Yarong, Lin Weibin, Xiao Weicheng, Zou Zhenzhuang, Cui Guozhen
School of Bioengineering, Zhuhai Campus of Zunyi Medical University, Zhuhai, China.
Department of Pediatrics, The Fifth Affiliated Hospital of Zunyi Medical University, Zunyi, China.
Front Nutr. 2025 May 27;12:1564177. doi: 10.3389/fnut.2025.1564177. eCollection 2025.
Liver disease is a global health issue requiring effective therapeutic interventions. Although the individual hepatoprotective properties of glutathione, extract, and vitamin C are well-documented, their combined effects remain to be elucidated.
This study aims to investigate the hepatoprotective potential of a functional food formula named GGV to mitigate acute liver injury induced in mice.
GGV was orally administered in a mouse model of carbon tetrachloride (CCl)-induced acute liver injury. Liver function was assessed by measuring serum and hepatic biomarkers. Gut microbiota composition and diversity were evaluated using 16S rRNA gene sequencing. Serum metabolomic profiling was conducted using UPLC-Q/TOF-MS.
GGV administration significantly ameliorated CCl-induced liver dysfunction, exhibiting greater efficacy than its individual components. Gut microbiota analysis revealed that GGV treatment restored the microbial diversity and composition disrupted by CCl exposure. Metabolomic profiling further indicated that GGV normalized phospholipid, fatty acid, and bile acid levels. Correlation analysis identified specific microbial genera associated with serum bile acid profiles, suggesting that the hepatoprotective effects of GGV are mediated through modulation of gut microbiota composition and metabolites. Taken together, these findings support the potential of GGV as a promising dietary intervention for promoting liver health through the liver-microbiota-gut axis.
肝脏疾病是一个全球性的健康问题，需要有效的治疗干预措施。尽管谷胱甘肽、提取物和维生素C各自的肝脏保护特性已有充分记录，但其联合作用仍有待阐明。
本研究旨在探究一种名为GGV的功能性食品配方减轻小鼠急性肝损伤的肝脏保护潜力。
在四氯化碳（CCl）诱导的急性肝损伤小鼠模型中口服给予GGV。通过测量血清和肝脏生物标志物评估肝功能。使用16S rRNA基因测序评估肠道微生物群的组成和多样性。使用超高效液相色谱-四极杆/飞行时间质谱（UPLC-Q/TOF-MS）进行血清代谢组学分析。
给予GGV可显著改善CCl诱导的肝功能障碍，其疗效优于其单个成分。肠道微生物群分析显示，GGV治疗恢复了因接触CCl而破坏的微生物多样性和组成。代谢组学分析进一步表明，GGV使磷脂、脂肪酸和胆汁酸水平正常化。相关性分析确定了与血清胆汁酸谱相关的特定微生物属，表明GGV的肝脏保护作用是通过调节肠道微生物群组成和代谢产物介导的。综上所述，这些发现支持了GGV作为一种有前景的饮食干预措施，通过肝脏-微生物群-肠道轴促进肝脏健康的潜力。
