Department of Chemistry, Umeå University, 90187 Umeå, Sweden.
J Agric Food Chem. 2022 Aug 10;70(31):9711-9721. doi: 10.1021/acs.jafc.2c02783. Epub 2022 Jul 27.
Green microalgae are attractive to food, pharmaceutical, and biofuel industries due to the promising and diverse properties of their intracellular components. In current biotechnological applications, however, clear bottlenecks are the cell disruption and cell harvesting steps. Challenges in both of these processes are directly linked to the properties of the microalgal cell wall. The aim of this study was to explore the cell wall compositions and morphologies of four Nordic microalgal strains ( (13-1), sp. (B2-2), , and sp. (3-4)) and their changes in relation to logarithmic and stationary growth phases. Transmission electron microscopy imaging enabled us to visualize the cell walls and to observe structural elements such as spines, microfibrillar hairs, or layers. Using cryogenic X-ray photoelectron spectroscopy, we quantified lipid, protein, and polysaccharide content of the outer surface of the microalgal cell wall in cultures. Fourier transform infrared spectroscopy highlighted changes between growth phases within the polysaccharide and protein fractions of the cell wall. Very prominent differences were observed in sugar and protein composition of the sp. (B2-2) cell wall compared to the cell walls of the other three Nordic strains using trimethylsilyl derivatization.
由于其细胞内成分具有有前景的多样性特性，绿色微藻受到食品、制药和生物燃料行业的关注。然而，在当前的生物技术应用中，细胞破碎和细胞收获步骤是明显的瓶颈。这两个过程中的挑战都与微藻细胞壁的特性直接相关。本研究旨在探索四种北欧微藻菌株（(13-1)、 sp. (B2-2)、 sp. (3-4) 和 sp. (3-4))的细胞壁组成和形态及其与对数和稳定生长阶段的关系。透射电子显微镜成像使我们能够可视化细胞壁，并观察到结构元素，如刺、微纤维毛或层。使用低温 X 射线光电子能谱，我们定量了培养物中微藻细胞壁外表面的脂质、蛋白质和多糖含量。傅立叶变换红外光谱强调了细胞壁多糖和蛋白质部分在生长阶段之间的变化。使用三甲基硅烷基衍生化，我们观察到 sp. (B2-2)细胞壁的糖和蛋白质组成与其他三种北欧菌株的细胞壁有很大的不同。
