Canelli Greta, Murciano Martínez Patricia, Austin Sean, Ambühl Mark E, Dionisi Fabiola, Bolten Christoph J, Carpine Roberta, Neutsch Lukas, Mathys Alexander
Laboratory of Sustainable Food Processing, ETH Zürich, Schmelzbergstrasse 9, 8092 Zürich, Switzerland.
Nestlé Research, Route du Jorat 57, 1000 Lausanne, Switzerland.
J Agric Food Chem. 2021 Feb 24;69(7):2226-2235. doi: 10.1021/acs.jafc.0c05032. Epub 2021 Feb 11.
Microalgae are attractive for the food and cosmetic industries because of their nutrient composition. However, the bioaccessibility and extractability of nutrients in microalgae are limited by the rigid and indigestible cell wall. The goal of this study is to explore the cell wall polysaccharides (CWPSs) composition and morphology in heterotrophic and biomasses during growth. Our results showed that glucose was the major component of CWPSs and exopolysaccharides in . CWPSs have a similar sugar profile in exponential and stationary phases, essentially composed of rhamnose and galactose. cell wall thickness increased from 82 nm in the exponential phase to 114 nm in the stationary phase and consisted of two main layers. cell wall was 133 nm thick and composed of several membranes surrounding thecal plates. Understanding of the microalgae cell wall helps developing a more efficient and targeted biorefinery approach.
微藻因其营养成分而对食品和化妆品行业具有吸引力。然而，微藻中营养物质的生物可及性和可提取性受到坚硬且难以消化的细胞壁的限制。本研究的目的是探索异养生长过程中微藻细胞壁多糖（CWPSs）的组成和生物质形态。我们的结果表明，葡萄糖是CWPSs和胞外多糖的主要成分。CWPSs在指数生长期和稳定期具有相似的糖谱，主要由鼠李糖和半乳糖组成。细胞壁厚度从指数生长期的82纳米增加到稳定期的114纳米，由两个主要层组成。细胞壁厚度为133纳米，由围绕鞘板的几层膜组成。对微藻细胞壁的了解有助于开发更高效、有针对性的生物精炼方法。
