Thomassen A R, Nielsen T T, Bagger J P, Henningsen P
Scand J Clin Lab Invest. 1987 May;47(3):293-300.
Comparison of whole blood and plasma aorto-coronary sinus concentration differences of glutamate and alanine were made before, during and after coronary sinus pacing in seven patients with normal and six patients with stenotic coronary arteries. Mean differences between duplicate analyses were greater in whole blood than plasma both of glutamate (7.5 +/- 5.8 vs. 3.3 +/- 3.0 mumol/l, p less than 0.001) and of alanine (7.9 +/- 7.0 vs. 3.8 +/- 3.4 mumol/l, p less than 0.001). Concentrations of glutamate were 3.4 and of alanine 1.4 times higher in whole blood than in plasma. Blood cells were calculated to be responsible for about 20% of glutamate and alanine blood exchanges across the heart. Plasma and whole blood fluxes were closely positively correlated (glutamate:r = 0.81, alanine:r = 0.88) and had always the same direction. Differences in myocardial exchanges of amino acids between the patients with and without coronary artery disease, as well as rapid changes during pacing, could be demonstrated in plasma analyses but were not significantly reflected in whole blood glutamate determinations. This seemed to be due to the greater variations in whole blood analysis. In conclusion, differences in aorto-coronary sinus plasma concentrations reflected, although underestimated, whole blood fluxes. Because of considerable gains in precision of analysis, plasma should be preferred to whole blood for evaluations of glutamate and alanine exchanges across the human heart.
对7名冠状动脉正常患者和6名冠状动脉狭窄患者在冠状窦起搏前、起搏期间和起搏后全血与血浆中谷氨酸和丙氨酸的主动脉 - 冠状窦浓度差异进行了比较。重复分析之间的平均差异在全血中比在血浆中更大，无论是谷氨酸（7.5±5.8对3.3±3.0μmol / l，p＜0.001）还是丙氨酸（7.9±7.0对3.8±3.4μmol / l，p＜0.001）。全血中谷氨酸浓度比血浆高3.4倍，丙氨酸浓度比血浆高1.4倍。经计算，血细胞约占心脏中谷氨酸和丙氨酸血液交换的20%。血浆和全血通量密切正相关（谷氨酸：r = 0.81，丙氨酸：r = 0.88），且方向始终相同。在血浆分析中可以证明有冠状动脉疾病和无冠状动脉疾病患者之间心肌氨基酸交换的差异，以及起搏期间的快速变化，但在全血谷氨酸测定中没有明显反映出来。这似乎是由于全血分析中变化较大。总之，主动脉 - 冠状窦血浆浓度差异虽然被低估，但反映了全血通量。由于分析精度有显著提高，在评估人心脏中谷氨酸和丙氨酸交换时，血浆应优于全血。
