Graduate School of Agriculture, Hokkaido University, Kita-ku, Sapporo 060-8589, Japan.
Japan Society for the Promotion of Science (JSPS), Chiyoda-ku, Tokyo 102-0083, Japan.
Int J Mol Sci. 2022 Jul 23;23(15):8120. doi: 10.3390/ijms23158120.
Expression of taste 2 receptor (T2R) genes, also known as bitter taste receptor genes, has been reported in a variety of tissues. The white adipose tissue of mice has been shown to express , , , , and , but the function of T2Rs in adipocytes remains unclear. Here, we show that fasting and stimulation by bitter compounds both increased expression in mouse white adipose tissue, and serum starvation and stimulation by bitter compounds both increased the expression of genes in 3T3-L1 adipocytes, suggesting that T2Rs have functional roles in adipocytes. RNA sequencing analysis of 3T3-L1 adipocytes stimulated by epicatechin, the ligand of Tas2r126, suggested that this receptor may play a role in the differentiation of adipocytes. Overexpression of in 3T3-L1 preadipocytes decreases fat accumulation after induction of differentiation and reduces the expression of adipogenic genes. Together, these results indicate that Tas2r126 may be involved in adipocyte differentiation.
味觉 2 受体（T2R）基因的表达，也被称为苦味受体基因，已在多种组织中报道。已经表明，小鼠的白色脂肪组织表达 、 、 、 、 ，但 T2R 在脂肪细胞中的功能尚不清楚。在这里，我们表明，禁食和苦味化合物的刺激均增加了小鼠白色脂肪组织中 的表达，而血清饥饿和苦味化合物的刺激均增加了 3T3-L1 脂肪细胞中 基因的表达，表明 T2R 在脂肪细胞中具有功能作用。用表儿茶素（Tas2r126 的配体）刺激 3T3-L1 脂肪细胞的 RNA 测序分析表明，该受体可能在脂肪细胞分化中发挥作用。在 3T3-L1 前脂肪细胞中转染 后，诱导分化后的脂肪积累减少，脂肪生成基因的表达降低。综上所述，这些结果表明 Tas2r126 可能参与脂肪细胞分化。
