Soybean Research Institute of Northeast Agricultural University/Key Laboratory of Soybean Biology of Chinese Education Ministry, Harbin 150030, China.
Key Laboratory of Soybean Molecular Design Breeding, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Harbin 150030, China.
Int J Mol Sci. 2022 Jul 24;23(15):8159. doi: 10.3390/ijms23158159.
Ethylene response factors (ERFs) are involved in biotic and abiotic stress; however, the drought resistance mechanisms of many ERFs in soybeans have not been resolved. Previously, we proved that enhances resistance to the pathogen in soybean. Here, we determined that is induced by 20% PEG-6000. Compared to the wild-type plants, soybean plants overexpressing (-OE) displayed increased drought tolerance which was characterized by milder leaf wilting, less water loss from detached leaves, smaller stomatal aperture, lower Malondialdehyde (MDA) content, increased proline accumulation, and higher Superoxide dismutase (SOD) and Peroxidase (POD) activities under drought stress, whereas plants with silenced through RNA interference were the opposite. Chromatin immunoprecipitation and dual effector-reporter assays showed that GmERF113 binds to the GCC-box in the promoter, activating expression directly. Overexpressing improved drought resistance in the composite soybean plants with transgenic hairy roots. RNA-seq analysis revealed that GmERF113 downregulates abscisic acid 8'-hydroxylase 3 (') and upregulates various drought-related genes. Overexpressing and increased the abscisic acid (ABA) content and reduced the expression of ' in transgenic soybean plants and hairy roots, respectively. These results reveal that the GmERF113-GmPR10-1 pathway improves drought resistance and affects the ABA content in soybean, providing a theoretical basis for the molecular breeding of drought-tolerant soybean.
乙烯应答因子（ERFs）参与生物和非生物胁迫；然而，许多大豆 ERF 的抗旱机制尚未解决。以前，我们证明了 增强了大豆对病原体 的抗性。在这里，我们确定 被 20%PEG-6000 诱导。与野生型植物相比，过量表达 的大豆植株表现出增强的耐旱性，其特征为叶片萎蔫程度较轻、离体叶片水分损失较少、气孔孔径较小、丙二醛（MDA）含量较低、脯氨酸积累增加以及超氧化物歧化酶（SOD）和过氧化物酶（POD）活性较高在干旱胁迫下，而通过 RNA 干扰沉默 的植株则相反。染色质免疫沉淀和双效应子报告基因检测表明，GmERF113 结合到 启动子中的 GCC 盒，直接激活 表达。过量表达 改善了具有转基因毛状根的复合大豆植株的耐旱性。RNA-seq 分析显示，GmERF113 下调脱落酸 8'-羟化酶 3（'）并上调各种与干旱相关的基因。过量表达 和 分别增加了转基因大豆植株和毛状根中的脱落酸（ABA）含量并降低了 '的表达。这些结果表明，GmERF113-GmPR10-1 途径提高了大豆的抗旱性并影响了 ABA 含量，为耐旱性大豆的分子育种提供了理论依据。
