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维持代谢能需求下饲养的生长猪的整体代谢物比较。

Comparison of Global Metabolite for Growing Pigs Fed at Metabolizable Energy Requirement for Maintenance.

作者信息

Liu Hu, Chen Yifan, Wang Wenhui, Jiang Zhaoning, Ma Xi, Wang Fenglai

机构信息

State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, China.

College of Animal Science and Technology, Hebei Agricultural University, Baoding, China.

出版信息

Front Vet Sci. 2022 Jul 8;9:917033. doi: 10.3389/fvets.2022.917033. eCollection 2022.

Abstract

Though the energy requirement for maintenance is an important part of net energy system, little is known of the metabolic characteristics of maintenance energy expenditure. This study was investigated the effect of feeding level at metabolizable energy requirement for maintenance (FLM) on plasma metabolites in growing pigs. Ten barrows (22.5 ± 0.5 kg BW) were kept in metabolism crates and catheterized in the precaval vein during adaptation period. Pigs were fed a corn-soybean meal diet at 782 kJ ME/kg BW·d during d 1 to 8 and then were refeeding at 2,400 kJ ME/kg BW·d on d 9. Plasma samples of each pig were collected by catheter on the morning of d 1, 3, 5, 7, 9, and 10, respectively, for metabolomics testing. Results showed that the concentration of plasma urea nitrogen decreased under FLM ( < 0.01) and increased significantly after refeeding ( < 0.01). The concentration of total cholesterol, high-density lipoprotein, low-density lipoprotein, and albumin in plasma were decreased significantly after refeeding ( < 0.01). Eleven identified compounds were up-regulated and six ones were down-regulated under FLM. In conclusion, the energy metabolism of growing pigs was relatively stable after 4 days of feeding at FLM.

摘要

尽管维持能量需求是净能量系统的重要组成部分,但对于维持能量消耗的代谢特征却知之甚少。本研究探讨了维持代谢能需求时的采食水平(FLM)对生长猪血浆代谢物的影响。10头公猪(体重22.5±0.5千克)在适应期被饲养在代谢笼中,并在前腔静脉插入导管。在第1至8天,给猪饲喂能量为782千焦代谢能/千克体重·天的玉米-豆粕型日粮,然后在第9天恢复饲喂能量为2400千焦代谢能/千克体重·天的日粮。分别在第1、3、5、7、9和10天早晨通过导管采集每头猪的血浆样本用于代谢组学检测。结果表明,FLM条件下血浆尿素氮浓度降低(<0.01),恢复饲喂后显著升高(<0.01)。恢复饲喂后,血浆中总胆固醇、高密度脂蛋白、低密度脂蛋白和白蛋白的浓度显著降低(<0.01)。在FLM条件下,有11种已鉴定的化合物上调,6种下调。总之,生长猪在FLM条件下采食4天后能量代谢相对稳定。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8753/9309205/4fd4e1527fa5/fvets-09-917033-g0001.jpg

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