Department of Cardiovascular Surgery, The Second Xiangya Hospital, Central South University, Changsha, China.
Clinical Center for Gene Diagnosis and Therapy, The Second Xiangya Hospital, Central South University, Changsha, China.
Front Public Health. 2022 Jul 11;10:899846. doi: 10.3389/fpubh.2022.899846. eCollection 2022.
is a non-tuberculous mycobacterium and is found everywhere in the environment. However, infection in human is extremely rare, especially in immunocompetent individuals. It is difficult to detect infection because its symptoms are non-specific, and routine diagnostic tests are less sensitive. It is also resistant to commonly used antibiotics. Here, we report the first case of infection after laparoscopic cholecystectomy in China.
A 55-year-old man was admitted because of repeated infections at multiple incision sites for more than 1 year. Although routine diagnostic test results were negative, metagenomic next-generation sequencing (mNGS) identified DNA sequences of in tissue samples from incision sites. The presence of was further confirmed by polymerase chain reaction and capillary electrophoresis. After 60 days of quadruple therapy with clarithromycin, moxifloxacin, rifampicin, and oxycycline, the patient's wound healed.
We believe the case findings contribute to the limited amount of knowledge about infection and raises awareness that this infection can result in poor wound healing, even in an immunocompetent host. Owing to a lack of early, precise diagnosis, it is difficult to treat infections. Based on our findings, mNGS is a sensitive diagnostic test for infections.
是一种非结核分枝杆菌，存在于环境中的各个角落。然而，人类感染这种细菌的情况极为罕见，尤其是在免疫功能正常的个体中。由于其症状不具特异性，且常规诊断检测的敏感性较低，因此这种感染很难被发现。它还对常用的抗生素具有耐药性。在此，我们报告了中国首例腹腔镜胆囊切除术后感染的病例。
一名 55 岁男性因 1 年多来多个切口部位反复感染而入院。尽管常规诊断检测结果为阴性，但宏基因组下一代测序（mNGS）在切口部位的组织样本中鉴定出了 的 DNA 序列。聚合酶链反应和毛细管电泳进一步证实了 的存在。经过 60 天的克拉霉素、莫西沙星、利福平、和强力霉素四联治疗后，患者的伤口愈合。
我们认为该病例的发现有助于增加对 感染的了解，提高人们对这种感染可导致免疫功能正常宿主伤口愈合不良的认识。由于早期、准确的诊断较为困难，因此这种感染的治疗也颇具难度。基于我们的发现，mNGS 是一种用于 感染的敏感诊断检测方法。
