Fujian Provincial Key Laboratory of Featured Biochemical and Chemical Materials, College of Chemistry and materials, Ningde Normal University, 1 Xueyuan Road, Ningde, Fujian, China.
Luminescence. 2022 Oct;37(10):1725-1732. doi: 10.1002/bio.4348. Epub 2022 Aug 7.
Developing artificial nanoparticles with peroxidase-like activity have attracted great interests because their catalytic activity is not easy to lose under harsh conditions compared with natural enzymes. In this work, the N-doped carbon dots (CDs) were prepared; it was found that the N-doped CDs showed high peroxidase-like catalytic activity towards the reaction system of hydrogen peroxide:3,3',5,5'-tetramethylbenzidine (TMB). The catalytic mechanism of the N-doped CDs was explored using electron spin resonance spectra, cyclic voltammetry methods and simulation of density functional theory calculations. This work can offer a new feasible method for synthesizing N-doped carbon quantum dots used as artificial peroxidases in biological and environment applications.
具有类过氧化物酶活性的人工纳米粒子的开发引起了极大的兴趣，因为与天然酶相比，它们的催化活性在恶劣条件下不易丧失。在这项工作中，制备了氮掺杂碳点（CDs）；研究发现，氮掺杂 CDs 对过氧化氢：3,3',5,5'-四甲基联苯胺（TMB）反应体系表现出高的类过氧化物酶催化活性。通过电子顺磁共振谱、循环伏安法和密度泛函理论计算模拟探讨了氮掺杂 CDs 的催化机理。这项工作为合成氮掺杂碳量子点作为生物和环境应用中的人工过氧化物酶提供了一种新的可行方法。
