Abad Z Gloria, Burgess Treena I, Redford Amanda J, Bienapfl John C, Srivastava Subodh, Mathew Reny, Jennings Krysta
United States Department of Agriculture-Animal and Plant Health Inspection Service Plant Protection and Quarantine (USDA-APHIS-PPQ) Science and Technology, Plant Pathogen Confirmatory Diagnostics Laboratory, Laurel, MD 20708, U.S.A.
Phytophthora Science and Management, Harry Butler Institute, Murdoch University, Perth, WA, 6150, Australia.
Plant Dis. 2023 Apr;107(4):987-998. doi: 10.1094/PDIS-02-22-0448-FE. Epub 2023 Apr 24.
, with 203 species, is a genus of high importance in agriculture worldwide. Here, we present the online resource "", developed to facilitate the correct identification of species of using the type specimens from the original descriptions wherever possible. emphasizes species of high economic impact and regulatory concern for the United States. presents an interactive Lucid key and a tabular key for 161 culturable species described as of May 2018, including 141 ex-types and 20 well-authenticated specimens. contains standard operating procedures for morphological and molecular characterization, as well as a glossary, image gallery, and numerous links. Each of the 161 factsheets includes access to nomenclature and morphological and molecular features, including sequences of the internal transcribed spacer ribosomal DNA, cytochrome C oxidase subunit I (barcoding genes), YPT1, β-tubulin, elongation factor 1a, L10, heat shock protein 90, and other genes. contains an innovative in silico BLAST and phylogenetic sequence analysis using NCBI. The mobile app, released in August 2021 (free for Android or iOS), allows users to take the Lucid key into the laboratory. is the first online identification tool based on the ex-types implemented for plant pathogens. In this article, we also include information for 21 new species and one hybrid described after the publication of , the status of the specimens of the types and ex-types at international herbaria and culture collections, and the status of genomes at the GenBank (currently 153 genome assemblies which correspond to 42 described species, including 16 ex-types). The effectiveness of the online resource and the content of this article could inspire other researchers to develop additional identification tools for other important groups of plant pathogens.
（该属）有203个物种，是全球农业中具有高度重要性的一个属。在此，我们展示在线资源“”，其开发目的是尽可能利用原始描述中的模式标本，以促进对（该属）物种的正确鉴定。（该资源）强调对美国具有高经济影响和监管关注的物种。（该资源）提供了一个交互式的Lucid检索表和一个表格检索表，用于截至2018年5月描述的161个可培养物种，包括141个模式种和20个经过充分验证的标本。（该资源）包含形态学和分子特征描述的标准操作程序，以及一个术语表、图像库和众多链接。161份情况说明书中的每一份都包括获取命名法以及形态学和分子特征的途径，包括内部转录间隔区核糖体DNA、细胞色素C氧化酶亚基I（条形码基因）、YPT1、β - 微管蛋白、延伸因子1a、L10、热休克蛋白90和其他基因的序列。（该资源）包含一项利用NCBI进行的创新型电子BLAST和系统发育序列分析。（该资源）的移动应用程序于2021年8月发布（安卓或iOS系统免费），允许用户将Lucid检索表带入实验室。（该资源）是首个基于模式种为植物病原体实施的在线鉴定工具。在本文中，我们还纳入了在（该资源）发布后描述的21个新物种和1个杂交种的信息、国际标本馆和培养物保藏中心模式标本和模式种标本的状态，以及GenBank中基因组的状态（目前有153个基因组组装，对应42个已描述物种，包括16个模式种）。（该资源）在线资源的有效性以及本文的内容可能会激励其他研究人员为其他重要的植物病原体群体开发更多的鉴定工具。
