Hirooka Yuuri, Villanueva Oscar, Ponomareva Ekaterina, Crane Bennett L, Peters Rick D, Ellouze Walid, Nguyen Hai D T
Department of Clinical Plant Science, Faculty of Bioscience and Applied Chemistry, Hosei University, Koganei, 184-8584, Tokyo, Japan Agriculture and Agri-Food Canada, Ottawa Research and Development Centre Ottawa Canada.
Agriculture and Agri-Food Canada, Ottawa Research and Development Centre, 960 Carling Avenue, Ottawa, Ontario, K1A 0C6, Canada Hosei University, Koganei Tokyo Japan.
IMA Fungus. 2025 Jun 10;16:e156195. doi: 10.3897/imafungus.16.156195. eCollection 2025.
Pethybr. & Laff. and Pethybr. are oomycetes that cause root rot diseases of multiple plant species, including serious diseases of potato. These two species of were originally reported in Ireland more than 100 years ago and are closely related phylogenetically and morphologically similar. Both species have wide host ranges and can hybridise with each other. In this study, we sequenced whole genomes of the ex-type strain of (CBS 113.19) and the authentic strain of (P6180). The genomes of the two isolates were assembled into 52.5 Mb and 74.7 Mb, respectively. A total of 11,654 protein-encoding genes were predicted for and 15,970 for . Phylogenomic analyses of 2012 single-copy orthologous genes and 99 BUSCO genes from the stramenopiles dataset confirmed that they are sister species and show that they belong to clade 8. The secretome analysis of P6180, CBS 418.71 and CBS 113.19 may provide information for future research on resistance-breeding targets and strategies for pathogen control. This genomic characterisation of the two species provides additional reference data that might be useful for future studies on genetic variation, pathogenicity and biological traits.
佩西布勒菌属（Pethybr. & Laff.）和佩西布勒菌（Pethybr.）是卵菌纲真菌，可引发多种植物的根腐病，包括马铃薯的严重病害。这两个菌种100多年前最初在爱尔兰被报道，在系统发育上关系密切，形态相似。两个菌种都有广泛的宿主范围，并且能够相互杂交。在本研究中，我们对佩西布勒菌（CBS 113.19）的模式菌株和佩西布勒菌（P6180）的正宗菌株进行了全基因组测序。两个分离株的基因组分别组装成52.5 Mb和74.7 Mb。预测佩西布勒菌有11,654个蛋白质编码基因，佩西布勒菌有15,970个。对来自不等鞭毛菌数据集的2012个单拷贝直系同源基因和99个BUSCO基因进行的系统基因组分析证实它们是姊妹种，并表明它们属于进化枝8。对佩西布勒菌P6180、佩西布勒菌CBS 418.71和佩西布勒菌CBS 113.19的分泌蛋白组分析可能为未来抗病育种目标研究和病原体控制策略提供信息。这两个佩西布勒菌菌种的基因组特征提供了额外的参考数据，可能对未来关于佩西布勒菌遗传变异、致病性和生物学特性的研究有用。
