通过流量调节提高鼠伤寒沙门氏菌侧向流动免疫分析检测的灵敏度。

Improving the sensitivity of lateral flow immunoassay for Salmonella typhimurium detection via flow-rate regulation.

机构信息

State Key Laboratory of Biobased Material and Green Papermaking, School of Food Science and Engineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China.

School of Bioengineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China.

出版信息

Food Chem. 2022 Dec 15;397:133756. doi: 10.1016/j.foodchem.2022.133756. Epub 2022 Jul 22.

DOI:10.1016/j.foodchem.2022.133756

PMID:35901614

Abstract

Application of the traditional immunochromatographic assay (ICGA) has been limited by its poor sensitivity. The objective of this study was to increase the sensitivity of the traditional ICGA. A dual-mode ICGA (D-M ICGA) was developed by combining a nanozyme-assisted signal-amplification strategy with a magnetic-nanoparticle-based flow-speed-control strategy. Salmonella typhimurium can be detected simultaneously based on color and magnetic signals in the detection area of the D-M ICGA strip. The calculated limits of detection of 50 cfu·mL and 75 cfu·mL in the color and magnetic modes, respectively, were approximately 1000 times lower than those of the traditional ICGA. The selectivity and practical applicability of the D-M ICGA were also confirmed in this study. The results prove that the D-M ICGA is an assay that could be used for Salmonella typhimurium detection and can be easily adapted to detect other pathogenic bacteria.

摘要

传统免疫胶体金渗滤法（ICGA）的应用受到其灵敏度低的限制。本研究旨在提高传统 ICGA 的灵敏度。通过将纳米酶辅助信号放大策略与基于磁性纳米颗粒的流速控制策略相结合，开发了一种双模式 ICGA（D-M ICGA）。在 D-M ICGA 条的检测区域内，可以基于颜色和磁信号同时检测鼠伤寒沙门氏菌。在颜色和磁模式下，计算出的检测限分别为 50 cfu·mL 和 75 cfu·mL，分别比传统 ICGA 低约 1000 倍。本研究还证实了 D-M ICGA 的选择性和实际适用性。结果证明，D-M ICGA 是一种可用于鼠伤寒沙门氏菌检测的检测方法，并且可以很容易地适应于检测其他病原菌。