Department of Pathology, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, China.
Department of Gastroenterology, Hospital of Chengdu University of Traditional Chinese Medicine, No. 39 Shi-er-qiao Road, Chengdu, 610072 Sichuan Province, China.
Dis Markers. 2022 Jul 19;2022:7313026. doi: 10.1155/2022/7313026. eCollection 2022.
Thermotherapy has been presented as a promising strategy to be used as an effective nonsurgical technique for colorectal carcinoma. Although this strategy presents several advantages, including low toxicity and high repeatability, thermotherapy often needs to be combined with other therapies because residual tumor cells that survive hyperthermal treatment often lead to relapse. In this study, we evaluated the effects of -elemene, which has been proven to have the potential to reverse chemotherapy drug resistance, on promoting the antitumor effects of hyperthermia. -elemene treatment significantly promoted apoptosis after 2 hours of hyperthermia treatment and blocked cell cycle phases at G1/G0. -elemene also significantly decreased colony formation and tumor formation abilities after hyperthermia treatment. -elemene treatment significantly decreased HSP70, but not HSP90 or HSP27, induced by hyperthermia treatment without disturbing HSP70 mRNA. It was also found that -elemene decreased phosphorylated ERK1/2 induced by hyperthermia. Regain of HSP70 reversed -elemene-mediated apoptosis, indicating that -elemene may induce apoptosis by decreasing HSP70. Moreover, -elemene treatment significantly decreased invasion capacity by decreasing the EMT, which was induced by hyperthermia treatment. Taken together, our results offer a potential strategy for CRC therapy via the combination of hyperthermia and -elemene.
热疗已被提出作为一种有前途的策略，可作为结直肠癌的一种有效非手术技术。尽管这种策略有几个优点，包括低毒性和高重复性，但热疗通常需要与其他疗法结合使用，因为在高热治疗后存活的残余肿瘤细胞往往会导致复发。在这项研究中，我们评估了 -榄香烯的效果，-榄香烯已被证明有潜力逆转化疗药物耐药性，以促进热疗的抗肿瘤作用。-榄香烯处理在 2 小时的热疗后显著促进细胞凋亡，并阻止细胞周期在 G1/G0 期。-榄香烯处理后还显著降低了集落形成和肿瘤形成能力。-榄香烯处理显著降低了 HSP70，但不降低 HSP90 或 HSP27，而不干扰 HSP70 mRNA。还发现 -榄香烯降低了热疗诱导的磷酸化 ERK1/2。HSP70 的恢复逆转了 -榄香烯介导的凋亡，表明 -榄香烯可能通过降低 HSP70 诱导细胞凋亡。此外，-榄香烯处理通过降低 EMT 显著降低了侵袭能力，这是由热疗诱导的。总之，我们的结果提供了一种通过热疗和 -榄香烯联合治疗 CRC 的潜在策略。
