WormRuler：一种用于追踪体长的软件，用于表征超红移通道视紫红质…… （原文此处表述不完整）

WormRuler: A software to track body length used to characterize a super red-shifted channelrhodopsin in .

作者信息

Seidenthal Marius, Vettkötter Dennis, Gottschalk Alexander

机构信息

Buchmann Institute for Molecular Life Sciences, Goethe-University, Max-von-Laue-Strasse 15, D-60438 Frankfurt, Germany.

Department of Biochemistry, Chemistry, and Pharmacy, Institute for Biophysical Chemistry, Goethe-University, Max-von-Laue-Strasse 9, D-60438 Frankfurt, Germany.

出版信息

MicroPubl Biol. 2022 Jul 19;2022. doi: 10.17912/micropub.biology.000607. eCollection 2022.

DOI:10.17912/micropub.biology.000607

PMID:35903772

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9315411/

Abstract

Manipulation of neuronal or muscular activity by optogenetics or other stimuli can be directly linked to the analysis of ( ) body length. Thus, WormRuler was developed as an open-source video analysis toolbox that offers video processing and data analysis in one application. Utilizing this novel tool, the super red-shifted channelrhodopsin variant, ChrimsonSA, was characterized in . Expression and activation of ChrimsonSA in GABAergic motor neurons results in their depolarization and therefore elongation of body length, the extent of which providing information about the strength of neuronal transmission.

摘要

通过光遗传学或其他刺激对神经元或肌肉活动进行操控，可以直接与（）体长的分析联系起来。因此，开发了WormRuler作为一个开源视频分析工具箱，该工具箱在一个应用程序中提供视频处理和数据分析。利用这个新颖的工具，在（）中对超红移视紫红质变体ChrimsonSA进行了表征。ChrimsonSA在GABA能运动神经元中的表达和激活导致其去极化，进而使体长延长，体长延长的程度提供了有关神经元传递强度的信息。