白皮杉醇对亚硒酸盐诱导的大鼠白内障及氧化损伤的保护作用。

Protective Effects of Piceatannol against Selenite-Induced Cataract and Oxidative Damage in Rats.

机构信息

Senior Department of Ophthalmology, The Third Medical Center of PLA General Hospital, Beijing, China.

出版信息

Curr Eye Res. 2022 Sep;47(9):1272-1278. doi: 10.1080/02713683.2022.2104320. Epub 2022 Jul 31.

DOI:10.1080/02713683.2022.2104320

PMID:35904449

Abstract

PURPOSE

This study aimed to investigate the protective effects of piceatannol (PIC) on selenite-induced cataracts in Sprague-Dawley rats and explore its therapeutic effects as an antioxidant.

METHODS

Thirty-two eight-day-old rat pups were randomly divided into four groups, with eight pups in each of them. Group 1, as the control group, was injected with the same amount of saline, while Groups 2-4 were administered with sodium selenite (3.46 mg/kg) subcutaneously into the neck on postpartum day 10 for cataract induction. Without further treatment, Group 2 served as the control model, while Groups 3 and 4 (low- and high-dose PIC-treated) had intraperitoneal piceatannol from day 8 to day 17 at doses of 10 mg/kg and 20 mg/kg, respectively. On postpartum day 17, after the last injection, the rat pups were examined for cataract grade by slit lamp, and the lenses of every group were isolated for oxidative damage indicators and further analysis. SRA01/04 cells were exposed to 600 μM HO for 24 hours with or without pretreatment with 10μМ piceatannol. Cell viability was tested by CCK-8 assay and cell apoptosis was evaluated by AnnexinV-PE/7AAD assay.

RESULTS

This study determined that compared with the model group, the degree of lens opacity was significantly reduced in PIC-treated groups. The histopathological damage of the lenses in the PIC-treated groups improved compared to the model group. There were fewer signs of lesions, such as vacuoles and atrophy. The biochemical results indicated that malondialdehyde (MDA) content of the PIC-treated groups were downregulated and the antioxidant enzyme activities (GSH and catalase) and antioxidant status (SOD) were upregulated compared with the model group. , piceatannol significantly restored cell viability and cell apoptosis under HO injury.

CONCLUSION

Pretreatment with piceatannol may achieve a protective effect on cataract development through upregulating antioxidant enzyme activity.

摘要

目的

本研究旨在探讨白藜芦醇（PIC）对亚硒酸钠诱导的 Sprague-Dawley 大鼠白内障的保护作用，并探讨其作为抗氧化剂的治疗效果。

方法

将 32 只 8 天大的幼鼠随机分为 4 组，每组 8 只。第 1 组作为对照组，注射等量生理盐水，第 2-4 组于产后第 10 天颈部皮下注射亚硒酸钠（3.46mg/kg）诱导白内障。不做进一步处理，第 2 组作为对照组模型，第 3 组和第 4 组（低剂量和高剂量 PIC 处理）分别于产后第 8 天至第 17 天腹腔内注射白藜芦醇 10mg/kg 和 20mg/kg。最后一次注射后，于产后第 17 天通过裂隙灯检查白内障分级，分离每组晶状体进行氧化损伤指标检测和进一步分析。SRA01/04 细胞用 600μM HO 处理 24 小时，并用或不用 10μM 白藜芦醇预处理。通过 CCK-8 测定法检测细胞活力，通过 AnnexinV-PE/7AAD 测定法评估细胞凋亡。

结果

本研究表明，与模型组相比，PIC 处理组晶状体混浊程度明显降低。与模型组相比，PIC 处理组晶状体的组织病理学损伤有所改善。病变迹象如空泡和萎缩减少。生化结果表明，与模型组相比，PIC 处理组丙二醛（MDA）含量下调，抗氧化酶活性（GSH 和过氧化氢酶）和抗氧化状态（SOD）上调。在 HO 损伤下，白藜芦醇显著恢复了细胞活力和细胞凋亡。