Doganay Selim, Borazan Mehmet, Iraz Mustafa, Cigremis Yilmaz
Department of Ophthalmology, Inonu University Medical Faculty, Turgut Ozal Medical Center, Malatya, Turkey. sdoganay2inonu.edu.tr
Curr Eye Res. 2006 Feb;31(2):147-53. doi: 10.1080/02713680500514685.
To investigate if resveratrol can prevent sodium selenite-induced experimental cataract model in rats.
Forty-eight Spraque-Dawley rat pups were divided into 3 treatment groups: (1) normal saline-% 5 ethanol injected i.p. on postpatum day 10; (2) Na selenite (30 nmol/g body wt) injected s.c on day 10; (3) Na selenite s.c on day 10+resveratrol (40 mg/kg) i.p on days 10-13. On day 21, cataract development was graded by slit-lamp examination and photography. Encapsulated lenses and erythrocytes were analyzed for reduced glutathione (GSH) and malondialdehyde (MDA), a marker of lipid peroxidation. Lenses were also analyzed for total nitrite (TN).
All control lenses in group 1 were clear. In group 2, all rats developed cataracts (grade 3-grade 6), whereas in group 3, only 9 of 16 rats developed cataracts (grade 2-grade 3). The difference of cataract frequency between groups 2 and 3 was statistically significant (p<0.05). Group 3 lenses and erythrocytes had higher mean GSH and lower mean MDA levels than those in group 2 (p<0.05). TN was highest in group 3 and lowest in group 1 (p<0.05).
Resveratrol suppressed selenite-induced oxidative stress and cataract formation in rats. This protective effect was supported by higher GSH and lower MDA in lens and erythrocytes. The presence of oxidative stress in selenite cataract development and its prevention by resveratrol support the possibility that high natural consumption of resveratrol in food can help prevent human senile cataract.
研究白藜芦醇是否能预防亚硒酸钠诱导的大鼠实验性白内障模型。
将48只Spraque-Dawley幼鼠分为3个治疗组：（1）在出生后第10天腹腔注射生理盐水-%5乙醇；（2）在第10天皮下注射亚硒酸钠（30 nmol/g体重）；（3）在第10天皮下注射亚硒酸钠，在第10至13天腹腔注射白藜芦醇（40 mg/kg）。在第21天，通过裂隙灯检查和摄影对白内障发展进行分级。分析晶状体囊膜和红细胞中的还原型谷胱甘肽（GSH）和脂质过氧化标志物丙二醛（MDA）。还分析晶状体中的总亚硝酸盐（TN）。
第1组所有对照晶状体均清晰。第2组所有大鼠均发生白内障（3级-6级），而第3组16只大鼠中只有9只发生白内障（2级-3级）。第2组和第3组之间白内障发生率的差异具有统计学意义（p<0.05）。第3组晶状体和红细胞的平均GSH水平高于第2组，平均MDA水平低于第2组（p<0.05）。TN在第3组最高，在第1组最低（p<0.05）。
白藜芦醇抑制了大鼠体内亚硒酸钠诱导的氧化应激和白内障形成。晶状体和红细胞中较高的GSH和较低的MDA支持了这种保护作用。亚硒酸钠白内障发展过程中氧化应激的存在及其被白藜芦醇预防，支持了食物中高天然白藜芦醇摄入量有助于预防人类老年性白内障的可能性。
