Characterization of Tremulane Sesquiterpene Synthase from the Basidiomycete .

Tremulane sesquiterpenoids are key secondary metabolites of the basidiomycete , which displays structural diversity and various bioactivities. However, tremulane sesquiterpene synthases have not been reported to date. The tremulane sesquiterpene synthase of was characterized by genome mining, heterologous expression, an assay, and substrate feeding. Moreover, the structures of the corresponding products were elucidated by NMR spectroscopy and X-ray diffraction analysis.