Wang Yue, Duan Yingce, Zhang Menghan, Liang Chaoqin, Li Wenli, Liu Chengwei, Ye Ying
Key Laboratory for Enzyme and Enzyme-Like Material Engineering of Heilongjiang, College of Life Science, Northeast Forestry University, Harbin 150040, China.
Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation, School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
J Fungi (Basel). 2024 Dec 7;10(12):846. doi: 10.3390/jof10120846.
is an edible and medicinal macrofungus with significant biological activity and broad pharmaceutical prospects that has received increasing attention in recent years. Although it is an important resource for macrofungi, knowledge of it remains limited. In this study, we sequenced, de novo assembled, and annotated the whole genome of using a PacBio Sequel II sequencer. The assembled 41.83 Mb genome contains 13,135 predicted protein-coding genes, 83.44% of which have searchable sequence similarity to other genes available in public databases. Using genome-based bioinformatics analysis, we identified 556 enzymes involved in carbohydrate metabolism and 103 cytochrome P450 proteins. Genome annotation revealed genes for key enzymes responsible for the biosynthesis of secondary metabolites, such as terpenoids and polyketides. Among them, we identified 14 terpene synthases, 8 NRPS-like enzymes, and 4 polyketide synthases (PKS), as well as 2 clusters of biosynthetic genes presumably related to terpene synthesis in . Gene family analysis revealed that the MYB transcription factor gene family plays an important role in the growth and development of . This study further enriches the genomic content of , provides genomic information for further research on the molecular mechanism of , and promotes the development of in the fields of drug research and functional food production.
是一种具有重要生物活性和广阔药用前景的食药用大型真菌，近年来受到越来越多的关注。尽管它是大型真菌的重要资源，但对其了解仍然有限。在本研究中，我们使用PacBio Sequel II测序仪对其全基因组进行了测序、从头组装和注释。组装得到的41.83 Mb基因组包含13135个预测的蛋白质编码基因，其中83.44%与公共数据库中其他可用基因具有可搜索的序列相似性。通过基于基因组的生物信息学分析，我们鉴定出556种参与碳水化合物代谢的酶和103种细胞色素P450蛋白。基因组注释揭示了负责次生代谢物生物合成的关键酶基因，如萜类化合物和聚酮化合物。其中，我们鉴定出14种萜烯合酶、8种类非核糖体肽合成酶和4种聚酮合酶（PKS），以及2个可能与萜烯合成相关的生物合成基因簇。基因家族分析表明，MYB转录因子基因家族在其生长发育中起重要作用。本研究进一步丰富了其基因组内容，为进一步研究其分子机制提供了基因组信息，并促进了其在药物研究和功能性食品生产领域的发展。
