Department of Biology, College of Science, University of Duhok, Duhok, Kurdistan Region, Iraq.
Directorate of Akre Education, General Directorate of Duhok Education, Duhok, Kurdistan Region, Iraq.
J Infect Dev Ctries. 2022 Jul 28;16(7):1126-1130. doi: 10.3855/jidc.15825.
In 2019, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the agent of Coronavirus Disease 2019 (COVID-19), spread all over the world. This global pandemic spread rapidly to more than 195 countries and caused over 200 million infections with a mortality rate of 2%. This study aimed to detect seropositivity against the SARS-CoV-2 virus among outpatients, symptomatic and asymptomatic individuals.
A total of 489 individuals of age 5-70 years (mean 38.0 ± 17 SD) were enrolled for a cross-sectional study. They were tested for presence of SARS-CoV-2 IgM/IgG antibodies in serum samples using Enzyme-linked Immunofluorescent Assay (ALFA).
A higher seroprevalence was recorded for IgM among females (34.96%) than males (28.83%). In the case of IgG, and IgG + IgM, both males and females had similar values. A significant correlation was identified between seropositivity and age; higher seropositivity (IgG, IgM, and IgG + IgM) was recorded in age groups 51-60 and ≥ 61 years, relative to the younger age groups. No significant correlation was found between symptomatic and asymptomatic individuals. No significant correlation was detected between seropositivity and RT-PCR positive and negative cases.
COVID-19 is spreading rapidly and there is a high percentage of asymptomatic carriers. The sensitivity of RT-PCR tests is not uniform and may not be able to detect all cases. On the other hand, serology can be used for large scale testing to detect the real extent to which the disease has spread.
2019 年，严重急性呼吸综合征冠状病毒 2（SARS-CoV-2），即 2019 年冠状病毒病（COVID-19）的病原体，在全球范围内传播。这种全球大流行迅速蔓延到 195 多个国家，导致超过 2 亿人感染，死亡率为 2%。本研究旨在检测门诊患者、有症状和无症状个体中针对 SARS-CoV-2 病毒的血清阳性率。
共纳入 489 名年龄在 5-70 岁的个体（平均年龄 38.0 ± 17 岁）进行横断面研究。使用酶联免疫荧光测定法（ALFA）检测血清样本中 SARS-CoV-2 IgM/IgG 抗体的存在。
女性（34.96%）的 IgM 血清阳性率高于男性（28.83%）。就 IgG 和 IgG+IgM 而言，男性和女性的数值相似。血清阳性率与年龄之间存在显著相关性；与年轻年龄组相比，51-60 岁和≥61 岁年龄组的 IgG、IgM 和 IgG+IgM 血清阳性率更高。无症状和有症状个体之间未发现显著相关性。血清阳性率与 RT-PCR 阳性和阴性病例之间未发现显著相关性。
COVID-19 正在迅速传播，有很大比例的无症状携带者。RT-PCR 检测的敏感性并不一致，可能无法检测到所有病例。另一方面，血清学可用于大规模检测，以发现疾病传播的真实程度。
