Otto Ferdinand, Sun Xing, Schulz Florian, Sanchez-Cano Carlos, Feliu Neus, Westermeier Fabian, Parak Wolfgang J
Center for Hybrid Nanostructures (CHyN), Universität Hamburg, Luruper Chaussee 149, 22761, Hamburg, Germany.
Hunan University, Lushan Road (S) 2, Changsha, 410012, P. R. China.
Small. 2022 Sep;18(37):e2201324. doi: 10.1002/smll.202201324. Epub 2022 Jul 29.
X-ray photon correlation spectroscopy (XPCS), a synchrotron source-based technique to measure sample dynamics, is used to determine hydrodynamic diameters of gold nanoparticles (Au NPs) of different sizes in biological environments. In situ determined hydrodynamic diameters are benchmarked with values obtained by dynamic light scattering. The technique is then applied to analyze the behavior of the Au NPs in a biological environment. First, a concentration-dependent agglomeration in the presence of NaCl is determined. Second, concentration-dependent increase in hydrodynamic diameter of the Au NPs upon the presence of proteins is determined. As X-rays in the used energy range are barely scattered by biological matter, dynamics of the Au NPs can be also detected in situ in complex biological environments, such as blood. These measurements demonstrate the possibility of XPCS for in situ analytics of nanoparticles (NPs) in biological environments where similar detection techniques based on visible light would severely suffer from scattering, absorption, and reflection effects.
X射线光子相关光谱法（XPCS）是一种基于同步加速器源来测量样品动力学的技术，用于测定生物环境中不同尺寸金纳米颗粒（Au NPs）的流体动力学直径。将原位测定的流体动力学直径与通过动态光散射获得的值进行基准对比。然后该技术被应用于分析金纳米颗粒在生物环境中的行为。首先，确定在氯化钠存在下浓度依赖性的团聚情况。其次，确定在蛋白质存在时金纳米颗粒流体动力学直径随浓度的增加情况。由于所用能量范围内的X射线几乎不会被生物物质散射，金纳米颗粒的动力学也可以在复杂的生物环境（如血液）中原位检测到。这些测量证明了XPCS在生物环境中原位分析纳米颗粒（NPs）的可能性，而基于可见光的类似检测技术在生物环境中会因散射、吸收和反射效应而严重受限。
