Brazilian Synchrotron Light Laboratory (LNLS), Brazilian Center for Research in Energy & Materials (CNPEM), Campinas, Sao Paulo 13083-970, Brazil.
Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA), Facultad de Ciencias Exactas, Universidad Nacional de La Plata - CONICET, 1900 La Plata, Argentina.
Nano Lett. 2024 Oct 23;24(42):13293-13299. doi: 10.1021/acs.nanolett.4c03662. Epub 2024 Oct 1.
In biological systems, nanoparticles interact with biomolecules, which may undergo protein corona formation that can result in noncontrolled aggregation. Therefore, comprehending the behavior and evolution of nanoparticles in the presence of biological fluids is paramount in nanomedicine. However, traditional lab-based colloid methods characterize diluted suspensions in low-complexity media, which hinders in-depth studies in complex biological environments. Here, we apply X-ray photon correlation spectroscopy (XPCS) to investigate silica nanoparticles (SiO) in various environments, ranging from low to high complex biological media. Interestingly, SiO revealed Brownian motion behavior, irrespective of the complexity of the chosen media. Moreover, the SiO surface and media composition were tailored to underline the differences between a corona-free system from protein corona and aggregates formation. Our results highlighted XPCS potential for real-time nanoparticle analysis in biological media, surpassing the limitations of conventional techniques and offering deeper insights into colloidal behavior in complex environments.
在生物系统中，纳米粒子与生物分子相互作用，这可能导致蛋白质冠形成，从而导致不可控的聚集。因此，了解纳米粒子在生物流体存在下的行为和演变在纳米医学中至关重要。然而，传统的基于实验室的胶体方法在低复杂性介质中对稀释悬浮液进行了表征，这阻碍了在复杂生物环境中的深入研究。在这里，我们应用 X 射线光子相关光谱学（XPCS）来研究不同环境中的二氧化硅纳米粒子（SiO），范围从低到高复杂的生物介质。有趣的是，SiO 表现出布朗运动行为，而不论所选介质的复杂性如何。此外，SiO 表面和介质组成经过了精心设计，以突出无冠状物系统与蛋白质冠状物和聚集物形成之间的差异。我们的结果突出了 XPCS 在生物介质中实时分析纳米粒子的潜力，超越了传统技术的局限性，并深入了解了复杂环境中的胶体行为。
