一种用于活细胞和体内比色法检测过氧亚硝酸盐的高选择性探针。
A highly selective probe for ratiometric imaging peroxynitrite in living cells and in vivo.
机构信息
College of Laboratory Medicine, Hebei Key Laboratory of Quality & Safety Analysis-Testing for Agro-Products and Food, Zhang Jiakou Key Laboratory of Organic Light Functional Materials, Hebei North University, Zhangjiakou 075000, Hebei Province, PR China.
Shandong Center for Food and Drug Evaluation and Inspection, Jinan, Shandong 250000, PR China; State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002, PR China.
出版信息
Bioorg Chem. 2022 Nov;128:106055. doi: 10.1016/j.bioorg.2022.106055. Epub 2022 Jul 22.
In this study, we constructed and displayed a ratiometric fluorescent probe JQ-2 for detecting ONOO. The probe JQ-2 showed a ratiometric signal for visualizing ONOO with a rapid response and high selectivity over a panel of biological analytes. Moreover, the JQ-2 has near-infrared emission (657 nm), which provides an excellent basis for the practical application in biological systems. The probe JQ-2 possessed low cytotoxicity and excellent cell membrane permeability, which can specifically visualize the exogenous and endogenous ONOO in vitro and vivo by emission in two channels. Meanwhile, JQ-2 can be used for diagnosing drug-induced liver injury by visualizing and monitoring the fluctuations of endogenous ONOO. Therefore, JQ-2 provided a potential tool for precisely detecting the fluctuation of ONOO in biological systems to understand physiological and pathological process.
在本研究中，我们构建并展示了用于检测 ONOO 的比率荧光探针 JQ-2。探针 JQ-2 对 ONOO 的可视化具有比率信号，对一系列生物分析物具有快速响应和高选择性。此外，JQ-2 具有近红外发射（657nm），这为其在生物系统中的实际应用提供了极好的基础。探针 JQ-2 具有低细胞毒性和优异的细胞膜通透性，可通过双通道发射特异性地可视化体外和体内的外源性和内源性 ONOO。同时，JQ-2 可用于通过可视化和监测内源性 ONOO 的波动来诊断药物性肝损伤。因此，JQ-2 为精确检测生物系统中 ONOO 的波动提供了一种潜在的工具，以了解生理和病理过程。
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