Department of Microbiology, Faculty of Pharmacy, The British University in Egypt (BUE), El-Sherouk City, 11837, Cairo, Egypt.
Department of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, African Union Organization Street, Abbasia, Cairo, 11566, Egypt.
Sci Rep. 2022 Jul 29;12(1):13048. doi: 10.1038/s41598-022-17275-z.
Strong biofilm-forming Enterococcus feacalis urinary tract pathogens (n = 35) were used to determine the lytic spectrum of six bacteriophages isolated from sewage samples. Only 17 Enterococcus feacalis isolates gave lytic zones with the tested bacteriophages from which five isolates were susceptible to all of them. The isolated enterococcal phages are characterized by wide range of thermal (30-90 °C) and pH (3-10) stability. They belong to order Caudovirales, from which four bacteriophages (EPA, EPB, EPD, EPF) belong to family Myoviridae and two (EPC, EPE) belong to family Siphoviridae. In addition, they have promising antibiofilm activity against the tested strong-forming biofilm E. faecalis isolates. The enterococcal phages reduced the formed and preformed biofilms to a range of 38.02-45.7% and 71.0-80.0%, respectively, as compared to the control. The same promising activities were obtained on studying the anti-adherent effect of the tested bacteriophages on the adherence of bacterial cells to the surface of urinary catheter segments. They reduced the number of adherent cells to a range of 30.8-43.8% and eradicated the pre-adherent cells to a range of 48.2-71.1%, as compared to the control. Overall, the obtained promising antibiofilm activity makes these phages good candidates for application in preventing and treating biofilm associated Enterococcus faecalis infections.
我们使用 35 株强生物膜形成粪肠球菌尿路感染病原体来确定从污水样本中分离的六种噬菌体的裂解谱。只有 17 株粪肠球菌分离株与测试的噬菌体产生裂解带，其中 5 株分离株对所有噬菌体均敏感。分离的肠球菌噬菌体具有较宽的热（30-90°C）和 pH（3-10）稳定性范围。它们属于长尾病毒目，其中 4 种噬菌体（EPA、EPB、EPD、EPF）属于肌尾病毒科，2 种（EPC、EPE）属于短尾病毒科。此外，它们对测试的强形成生物膜粪肠球菌分离株具有有前景的抗生物膜活性。与对照组相比，肠球菌噬菌体将形成和预形成的生物膜减少到 38.02-45.7%和 71.0-80.0%的范围。在研究测试噬菌体对细菌细胞粘附到导尿管段表面的粘附抑制作用时，获得了相同的有前景的活性。与对照组相比，它们将粘附细胞的数量减少到 30.8-43.8%的范围，并将预粘附细胞消除到 48.2-71.1%的范围。总的来说，获得的有前景的抗生物膜活性使这些噬菌体成为预防和治疗与生物膜相关的粪肠球菌感染的良好候选物。
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