University of South Australia, Adelaide, Australia.
Curr Microbiol. 2010 Jun;60(6):400-6. doi: 10.1007/s00284-009-9555-z. Epub 2009 Dec 5.
Most of the bacteriophages (phages) currently reported in Enterococcus spp. belong to tailed families of bacteriophages Podoviridae, Siphoviridae, and Myoviridae. There is a little information on non-tailed bacteriophages isolated from enterococci. Samples of sewage and piggery effluents were tested on pig and chicken isolates of Enterococcus faecalis, E. faecium and E. gallinarum for lytic phages. In addition, isolates were exposed to mitomycin C to induce lysogenic phages. Bacteriophages that were detected were visualized by electron microscopy. Ten bacteriophages were of isometric shape with long flexible or non-flexible tails, while one had a long head with a long flexible tail; all contained double-stranded DNA molecules. Seven Polyhedral, filamentous, and pleomorphic-shaped phages containing DNA or RNA were also observed. The pleomorphic phages were droplet- or lemon-shaped in morphology. This study is the first report on polyhedral phages in Enterococcus spp. of animal origin and also the first report of filamentous and pleomorphic phages in enterococci.
目前在肠球菌属中报道的大多数噬菌体（phages）属于有尾噬菌体科的 Podoviridae、Siphoviridae 和 Myoviridae。关于从肠球菌中分离出的非尾噬菌体的信息较少。对来自猪和鸡的粪肠球菌、屎肠球菌和鸡肠球菌的分离物进行了噬菌活性测试，检测了污水和养猪场废水样本中的噬菌。此外，还将分离物暴露于丝裂霉素 C 以诱导溶原性噬菌体。通过电子显微镜观察到检测到的噬菌体。有 10 种噬菌体呈等轴状，带有长而灵活或不灵活的尾巴，而有一种噬菌体带有长头和长而灵活的尾巴；所有噬菌体都含有双链 DNA 分子。还观察到 7 种多面体、丝状和多形噬菌体，它们含有 DNA 或 RNA。多形噬菌体在形态上呈滴状或柠檬状。本研究首次报道了动物源肠球菌属中的多面体噬菌体，也是首次报道了肠球菌中的丝状和多形噬菌体。
