Department of Biology, College of Science, Mustansiriyah University, Palestine Street, PO Box 14022, Baghdad, Iraq.
Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.
Biotechnol Lett. 2022 Mar;44(3):513-522. doi: 10.1007/s10529-022-03229-y. Epub 2022 Feb 5.
The degradation activity of two bacteriophages UPMK_1 and UPMK_2 against methicillin-resistant Staphylococcus aureus phages were examined using gel zymography.
The analysis was done using BLASTP to detect peptides catalytic domains. Many peptides that are related to several phage proteins were revealed.
UPMK_1 and UPMK_2 custom sequence database were used for peptide identification. The biofilm-degrading proteins in the bacteriophage UPMK_2 revealed the same lytic activity towards polysaccharide intercellular adhesin-dependent and independent of Methicillin-resistant Staphylococcus aureus (MRSA) biofilm producers in comparison to UPMK_1, which had lytic activity restricted solely to its host.
Both bacteriophage enzymes were involved in MRSA biofilm degradation during phage infection and they have promising enzybiotics properties against MRSA biofilm formation.
使用凝胶电泳酶谱法检测两种噬菌体 UPMK_1 和 UPMK_2 对耐甲氧西林金黄色葡萄球菌噬菌体的降解活性。
使用 BLASTP 进行分析，以检测肽催化结构域。揭示了许多与几种噬菌体蛋白相关的肽。
使用 UPMK_1 和 UPMK_2 自定义序列数据库进行肽鉴定。与 UPMK_1 相比，噬菌体 UPMK_2 中的生物膜降解蛋白对多聚糖细胞间黏附素有相同的溶菌活性，并且不依赖于耐甲氧西林金黄色葡萄球菌（MRSA）生物膜生成菌。而 UPMK_1 仅对其宿主具有溶菌活性。
两种噬菌体酶都参与了噬菌体感染期间的 MRSA 生物膜降解，并且它们具有针对 MRSA 生物膜形成的有前途的酶类抗生素特性。
