College of Materials Science and Engineering, Wuhan Textile University, Wuhan 430200, China.
Key Laboratory of Green Processing and Functional New Textile Materials of Ministry of Education, Wuhan Textile University, Wuhan 430200, China; Institute for Frontier Materials, Deakin University, Geelong, Victoria 3216, Australia.
Int J Biol Macromol. 2022 Sep 1;216:810-819. doi: 10.1016/j.ijbiomac.2022.07.207. Epub 2022 Jul 29.
Biodegradable porous scaffolds with different structure, porosity, and strength play a critical role in the repair and regeneration of defects in bone tissue engineering by changing the proliferation condition for cell. In this study, polylactic acid (PLA) scaffold with directional porous structure is designed and fabricated using the method of ice template and phase inversion for speeding up bone repair by promoting the growth and proliferation of bone cells. The morphology, mechanical properties, hydrophilicity, and wicking properties of PLA scaffolds were characterized by scanning electron microscope, universal testing machine, contact angle tester and wicking rate test, respectively. In vitro biocompatibility has been investigated through measuring cell adhesion, proliferation, and viability on PLA scaffold with directional porous structure. Prepared PLA scaffold was implanted into animals to observe the repair mechanism of large-sized bone defects. This study proposes a novel bioporous scaffold design to induce osteocyte growth at the rat calvaria with a directional pore structure, and the scaffold edges were integrated with the calvaria at week 12, effectively promoting the repair and regeneration of defective bone tissue.
具有不同结构、孔隙率和强度的可生物降解多孔支架通过改变细胞的增殖条件，在骨组织工程中对缺陷的修复和再生起着关键作用。在这项研究中，采用冰模板法和相转化法设计并制备了具有定向多孔结构的聚乳酸（PLA）支架，通过促进骨细胞的生长和增殖来加速骨修复。通过扫描电子显微镜、万能试验机、接触角测试仪和吸液率测试分别对 PLA 支架的形态、力学性能、亲水性和吸液性能进行了表征。通过测量细胞在定向多孔结构 PLA 支架上的黏附、增殖和活力，研究了 PLA 支架的体外生物相容性。将制备的 PLA 支架植入动物体内，观察大尺寸骨缺损的修复机制。本研究提出了一种新的生物多孔支架设计，以诱导大鼠颅骨中成骨细胞的定向生长，具有定向孔结构，支架边缘在第 12 周与颅骨整合，有效促进了缺损骨组织的修复和再生。
