Charles River Discovery Research Services Germany GmbH, Freiburg, Germany.
Bioengineering Division, Draper, Cambridge, MA, USA.
Drug Discov Today. 2022 Oct;27(10):103329. doi: 10.1016/j.drudis.2022.07.014. Epub 2022 Jul 28.
During the past 15 years, a plethora of innovative 3D in vitro systems has been developed. They offer the possibility of identifying crucial cellular and molecular contributors to the disease by permitting manipulation of each in isolation. However, improvements are needed particularly with respect to the predictivity and validity of those models. The major challenge now is to identify which assay and readout combination(s) best suits the current scientific question(s). A deep understanding of the different platforms along with their pros and cons is a prerequisite to make this decision. This review aims to give an overview of the most prominent systems with a focus on applications, translational relevance and adoption drivers from an industry perspective.
在过去的 15 年中，已经开发出了大量创新性的 3D 体外系统。它们通过允许对每个系统进行单独操作，为确定疾病的关键细胞和分子贡献提供了可能性。然而，这些模型的预测性和有效性仍需要改进。目前的主要挑战是确定哪种检测和读数组合最适合当前的科学问题。深入了解不同平台及其优缺点是做出这一决策的前提。本文旨在对最主要的系统进行概述，重点从工业角度介绍它们的应用、转化相关性和采用驱动力。
