Hu Wencheng, Lin Junqing, Wei Jiabao, Yang Yunlong, Fu Kai, Zhu Tianhao, Zhu Hongyi, Zheng Xianyou
Department of Orthopaedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.
Institute of Microsurgery on Extremities, Department of Orthopedic Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.
Bone Joint Res. 2022 Aug;11(8):518-527. doi: 10.1302/2046-3758.118.BJR-2021-0575.R1.
To evaluate inducing osteoarthritis (OA) by surgical destabilization of the medial meniscus (DMM) in mice with and without a stereomicroscope.
Based on sample size calculation, 70 male C57BL/6 mice were randomly assigned to three surgery groups: DMM aided by a stereomicroscope; DMM by naked eye; or sham surgery. The group information was blinded to researchers. Mice underwent static weightbearing, von Frey test, and gait analysis at two-week intervals from eight to 16 weeks after surgery. Histological grade of OA was determined with the Osteoarthritis Research Society International (OARSI) scoring system.
Surgical DMM with or without stereomicroscope led to decrease in the mean of weightbearing percentages (-20.64% vs -21.44%, p = 0.792) and paw withdrawal response thresholds (-21.35% vs -24.65%, p = 0.327) of the hind limbs. However, the coefficient of variation (CV) of weight-bearing percentages and paw withdrawal response thresholds in naked-eye group were significantly greater than that in the microscope group (19.82% vs 6.94%, p < 0.001; 21.85% vs 9.86%, p < 0.001). The gait analysis showed a similar pattern. Cartilage degeneration was observed in both DMM-surgery groups, evidenced by increased OARSI scores (summed score: 11.23 vs 11.43, p = 0.842), but the microscope group showed less variation in OARSI score than the naked-eye group (CV: 21.03% vs 32.44%; p = 0.032).
Although surgical DMM aided by stereomicroscope is technically difficult, it produces a relatively more homogeneous OA model in terms of the discrete degree of pain behaviours and histopathological grading when compared with surgical DMM without stereomicroscope.Cite this article: 2022;11(8):518-527.
评估在有和没有体视显微镜的情况下，通过手术使内侧半月板失稳（DMM）诱导小鼠骨关节炎（OA）。
基于样本量计算，将70只雄性C57BL/6小鼠随机分为三个手术组：体视显微镜辅助下的DMM组；肉眼直视下的DMM组；或假手术组。研究人员对分组信息不知情。术后8至16周，每隔两周对小鼠进行静态负重、von Frey测试和步态分析。采用国际骨关节炎研究协会（OARSI）评分系统确定OA的组织学分级。
无论有无体视显微镜，手术DMM均导致后肢平均负重百分比降低（-20.64%对-21.44%，p = 0.792）和爪退缩反应阈值降低（-21.35%对-24.65%，p = 0.327）。然而，肉眼组负重百分比和爪退缩反应阈值的变异系数（CV）显著高于显微镜组（19.82%对6.94%，p < 0.001；21.85%对9.86%，p < 0.001）。步态分析显示出类似模式。在两个DMM手术组中均观察到软骨退变，OARSI评分升高证明了这一点（总分：11.23对11.43，p = 0.842），但显微镜组的OARSI评分变异小于肉眼组（CV：21.03%对32.44%；p = 0.032）。
尽管体视显微镜辅助下的手术DMM技术难度较大，但与无体视显微镜的手术DMM相比，在疼痛行为离散程度和组织病理学分级方面，它能产生相对更均匀的OA模型。引用本文：2022；11(8):518-527。
