Mobley C Brooks, Vechetti Ivan J, Valentino Taylor R, McCarthy John J
Department of Physiology, College of Medicine, University of Kentucky, Lexington, Kentucky.
J Appl Physiol (1985). 2020 May 1;128(5):1227-1239. doi: 10.1152/japplphysiol.00021.2020. Epub 2020 Feb 27.
The development of tissue-specific inducible transgenic mice has provided a powerful tool to study gene function and cell biology in almost any tissue of interest at any given time within the animal's life. The purpose of this review is to describe how to use two different inducible transgenic systems, the Cre-loxP system and the Tet-ON/OFF system, that can be used to study skeletal muscle physiology. Myofiber- and satellite cell-specific Cre-loxP transgenic mice are described as is how these mice can be used to knockout a gene of interest or to deplete satellite cells in adult skeletal muscle, respectively. A myofiber-specific Tet-ON system is described as is how such mice can be used to overexpress a gene of interest or to label myonuclei. How to effectively breed and genotype the transgenic mice are also described in detail. The hope is this review will provide the basic information necessary to facilitate the incorporation of tissue-specific inducible transgenic mice into a skeletal muscle research program.
组织特异性诱导型转基因小鼠的发展为在动物生命中的任何特定时间研究几乎任何感兴趣组织中的基因功能和细胞生物学提供了一个强大的工具。本综述的目的是描述如何使用两种不同的诱导型转基因系统，即Cre-loxP系统和Tet-ON/OFF系统，来研究骨骼肌生理学。描述了肌纤维和卫星细胞特异性Cre-loxP转基因小鼠，以及如何分别使用这些小鼠在成年骨骼肌中敲除感兴趣的基因或耗尽卫星细胞。描述了一种肌纤维特异性Tet-ON系统，以及如何使用此类小鼠过表达感兴趣的基因或标记肌核。还详细描述了如何有效地繁殖转基因小鼠并进行基因分型。希望本综述将提供必要的基本信息，以促进将组织特异性诱导型转基因小鼠纳入骨骼肌研究计划。
