Li Mingzhu, Yuan Yuanyuan, Ni Fei, Li Xingfeng, Wang Honggang, Bao Yinguang
State Key Laboratory of Crop Biology, Agronomy College of Shandong Agricultural University, Tai'an, China.
Bureau of Agriculture and Rural Affairs of Linqing, Liaocheng, China.
Front Plant Sci. 2022 Jul 15;13:943669. doi: 10.3389/fpls.2022.943669. eCollection 2022.
Powdery mildew is one of the most devastating foliar diseases in wheat production. The wild relative (2 = 10 = 70) has been widely used in wheat genetic improvement due to its superior resistance to both biotic and abiotic stresses. In the present study, two wheat- introgression lines named SN0293-2 and SN0293-7 were developed from the progenies of a cross between the octoploid SNTE20 and common wheat, including the elite cultivar Jimai 22. They had a novel powdery mildew resistance gene (temporarily named ) putatively from pyramided with and , exhibiting excellent resistance at both the seedling and adult stages. Sequential GISH-FISH detected no signal of in these two lines but a pair of T1BL·1RS in SN0293-2. Chromosomal structural variations were also observed obviously in SN0293-2 and SN0293-7. Through the Wheat 660K SNP array, 157 SNPs, 134 of which were on 6A, were found to be specific to . Based on the data combined with DNA re-sequencing, seven specific markers, including one CAPS marker on 2B and six CAPS and Indel markers on 6A, were developed, confirming their wheat- introgression nature. Furthermore, the two lines displayed positive plant height and produced more kernels and higher 1,000-grain weight. Excellent resistance with desirable agronomic traits makes them valuable in wheat breeding programs.
白粉病是小麦生产中最具毁灭性的叶部病害之一。野生近缘种（2n = 10 = 70）因其对生物和非生物胁迫均具有优异抗性，已被广泛用于小麦遗传改良。在本研究中，从八倍体SNTE20与普通小麦（包括优良品种济麦22）杂交后代中培育出两个小麦渐渗系，分别命名为SN0293 - 2和SN0293 - 7。它们含有一个推测来自的新型抗白粉病基因（暂命名），该基因与和聚合，在幼苗期和成年期均表现出优异的抗性。连续的基因组原位杂交 - 荧光原位杂交（GISH - FISH）检测发现这两个品系中没有的信号，但在SN0293 - 2中检测到一对T1BL·1RS。在SN0293 - 2和SN0293 - 7中也明显观察到染色体结构变异。通过小麦660K SNP芯片，发现157个SNP，其中134个位于6A染色体上，是特有的。基于这些数据并结合DNA重测序，开发了7个特异性标记，包括2B染色体上的1个CAPS标记和6A染色体上的6个CAPS和Indel标记，证实了它们的小麦渐渗特性。此外，这两个品系株高表现良好，籽粒较多，千粒重较高。优异的抗性和理想的农艺性状使其在小麦育种计划中具有重要价值。
