Li Mingzhu, Wang Yanzhen, Liu Xiaojuan, Li Xingfeng, Wang Honggang, Bao Yinguang
State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, China.
Agronomy College of Shandong Agricultural University, Tai'an, China.
Front Plant Sci. 2021 Oct 1;12:727734. doi: 10.3389/fpls.2021.727734. eCollection 2021.
(2 = 10 = 70) is a wild relative of wheat with high tolerance to both biotic and abiotic stresses; it has been wildly used in wheat genetic improvement. A disomic substitution line named SN19647 was derived from a cross between and the wheat- partial amphiploid SNTE20 (2 = 8 = 56). It was evaluated for disease resistance and characterized sequential fluorescence hybridization (FISH)-genomic hybridization (GISH) and molecular markers. The results showed that SN19647 carried resistance to both powdery mildew and leaf rust. It contained 42 chromosomes with a pair of wheat chromosome 1B replaced by a pair of J chromosomes from . In addition to chromosomal substitution events, structural variation also occurred on wheat chromosomes 2A, 5A, 6B, and 7B. Based on marker analysis, 19 markers specific to the J chromosome were obtained, of which seventeen markers belonged to homoeologous group one. These results indicated that SN19647 was a 1J (1B) substitution line. Compared with the known 1J (1D) substitution line CH10A5, it was found that 17 markers generated different specific bands to , confirming the novelty of the 1J chromosome in SN19647. Therefore, SN19647, resistant to powdery mildew and leaf rust, was a novel 1J (1B) substitution line that can be used in wheat genetic improvement.
（2n = 10 = 70）是小麦的野生近缘种，对生物和非生物胁迫均具有高耐受性；它已被广泛用于小麦遗传改良。一个名为SN19647的二体代换系源自（2n = 10 = 70）与小麦-部分双二倍体SNTE20（2n = 8x = 56）的杂交。对其进行了抗病性评估，并通过顺序荧光原位杂交（FISH）-基因组原位杂交（GISH）和分子标记进行了鉴定。结果表明，SN19647对白粉病和叶锈病均具有抗性。它含有42条染色体，其中一对小麦1B染色体被一对来自（2n = 10 = 70）的J染色体所取代。除了染色体代换事件外，小麦2A、5A、6B和7B染色体上也发生了结构变异。基于标记分析，获得了19个J染色体特异的标记，其中17个标记属于同源群一。这些结果表明SN19647是一个1J（1B）代换系。与已知的1J（1D）代换系CH10A5相比，发现17个标记对（2n = 10 = 70）产生了不同的特异条带，证实了SN19647中1J染色体的新颖性。因此，对白粉病和叶锈病具有抗性的SN19647是一个可用于小麦遗传改良的新型1J（1B）代换系。
