Paredes María Gimena, Bianco Karina Alesia, Menéndez-Helman Renata J, Kristoff Gisela
Laboratorio de Evaluación Ecotoxicológica del Agua: Invertebrados Nativos y Otros Modelos, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN)-CONICET, Universidad de Buenos Aires, Buenos Aires, Argentina.
Laboratorio de Enzimología, Estrés Oxidativo y Metabolismo, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN)-CONICET, Universidad de Buenos Aires, Buenos Aires, Argentina.
Front Physiol. 2022 Jul 14;13:954868. doi: 10.3389/fphys.2022.954868. eCollection 2022.
Lugano Lake is located in an Ecological Reserve of Buenos Aires City. Biomonitoring of its water quality is essential due to its importance as a place for recreation and protection of native species. is a native hermaphrodite aquatic gastropod that inhabits different freshwater bodies of Argentina and was recently selected as a potential bioindicator. We propose this study as a first approach to assessing specific organisms' use in biomonitoring of urban wild reserves, and the usefulness of reproduction assays. survival, behavior, reproduction success and offspring survival after the exposure to water samples from Lugano Lake (L1, L2, and L3) were evaluated. Temperature, pH, conductivity and dissolved oxygen were registered . Samples were transported to the laboratory and chemical analysis and bioassays were performed using 20 snails per site. A control group with tap water was added. Egg masses were separated, exposed individually and observed daily using a stereoscopic microscope. After hatching, juveniles were placed in tap water and offspring survival was registered at the first, second, third and fourth months after the beginning of the assay. High levels of conductivity, turbidity and nutrients were obtained. Ammonium and nitrite were higher than the guideline level for the protection of aquatic life. During the bioassay 20% of the snails (L2 and L3) showed abnormally protruding of the head-food region. The number of eggs and embryonated eggs per mass did not differ between treatments. Egg masses exposed to water samples from the lake presented overlapping and abnormal eggs and arrested embryos. Besides, low % of hatching (L1: 33%, L2: 42%, and L3: 16%) and juvenile survival after the first (L1:14%; L2:78%) and second month (L1: 60%) were noted. In the control group, 85% of hatching and 100%-90% of survival were observed. Our results suggests the presence of pollutant in the lake. seems to be a sensitive local species. spp. reproduction assays can provide a valuable endpoint for toxicity and risk assessments and a usefulness tool for biomonitoring water quality.
卢加诺湖位于布宜诺斯艾利斯市的一个生态保护区内。鉴于其作为休闲场所和本地物种保护地的重要性，对其水质进行生物监测至关重要。 是一种本地雌雄同体的水生腹足纲动物，栖息于阿根廷的不同淡水水体中，最近被选为潜在的生物指示物。我们提出本研究作为评估特定生物在城市野生保护区生物监测中的应用以及繁殖试验有用性的初步方法。评估了暴露于卢加诺湖（L1、L2和L3）水样后的 生存、行为、繁殖成功率和后代存活率。记录了温度、pH值、电导率和溶解氧。将样品运至实验室，每个采样点使用20只蜗牛进行化学分析和生物测定。添加了一个用自来水的对照组。将卵块分开，单独暴露，并每天使用立体显微镜观察。孵化后，将幼体置于自来水中，并在试验开始后的第一、第二、第三和第四个月记录后代存活率。获得了高电导率、浊度和营养物水平。铵和亚硝酸盐高于保护水生生物的指导水平。在生物测定过程中，20%的蜗牛（L2和L3）头部食物区域出现异常突出。各处理间每块卵的数量和受精卵数量无差异。暴露于湖水样的卵块出现重叠和异常的卵以及胚胎发育停滞。此外，还注意到孵化率低（L1：33%，L2：42%，L3：16%）以及第一个月（L1：14%；L2：78%）和第二个月（L1：60%）后的幼体存活率低。在对照组中，观察到85%的孵化率和100%-90%的存活率。我们的结果表明该湖存在污染物。 似乎是一种敏感的本地物种。 属繁殖试验可为毒性和风险评估提供有价值的终点指标，是水质生物监测的有用工具。
