Laboratorio de Evaluación Ecotoxicológica del Agua: Invertebrados Nativos Y Otros Modelos (EEAINM), Departamento de Química Biológica, Facultad de Ciencias Exactas Y Naturales, Instituto de Química Biológica de La Facultad de Ciencias Exactas Y Naturales (IQUIBICEN), Universidad de Buenos Aires, CONICET-Universidad de Buenos Aires, Buenos Aires, Argentina.
Laboratorio de Enzimología, Estrés Oxidativo Y Metabolismo (LEEM), Departamento de Química Biológica, Facultad de Ciencias Exactas Y Naturales, Instituto de Química Biológica de La Facultad de Ciencias Exactas Y Naturales (IQUIBICEN), Universidad de Buenos Aires, CONICET-Universidad de Buenos Aires, Buenos Aires, Argentina.
Bull Environ Contam Toxicol. 2023 Jun 7;110(6):108. doi: 10.1007/s00128-023-03742-4.
The study of multiple biomarkers in bioindicator species is a useful tool to evaluate water quality in addition to physicochemical analysis. The aim of this work was to study the toxicity of water samples from two sites with different anthropogenic impacts (R: near a residential area and FP: close to horticultural farms and industrial waste treatment plants) from Las Catonas sub-basin (Reconquista River basin) in the native gastropod Biomphalaria straminea. Some physicochemical parameters and chlorpyrifos concentration were measured in water samples. Snails were exposed in laboratory conditions 48 h to the water samples and neurotoxicity, behavior, lethality and acetylcholinesterase, carboxylesterase, glutathione S-transferase, glutathione reductase and catalase activities were measured. In water from FP, chlorpyrifos was detected and conductivity and pH were higher than in R. Lethality (60%) and a decrease (30%) in acetylcholinesterase were observed in snails exposed to FP indicating that water contamination causes high toxicity in B. straminea.
在生物指示剂物种中研究多种生物标志物是评估水质的有用工具，除了理化分析之外。本工作的目的是研究来自两个具有不同人为影响的地点（R：靠近居民区和 FP：靠近园艺农场和工业废物处理厂）的水样对 Las Catonas 亚流域（Reconquista 河流域）本地腹足纲动物 B. straminea 的毒性。在水样中测量了一些理化参数和毒死蜱浓度。蜗牛在实验室条件下暴露于水样中 48 小时，并测量了神经毒性、行为、致死率以及乙酰胆碱酯酶、羧酸酯酶、谷胱甘肽 S-转移酶、谷胱甘肽还原酶和过氧化氢酶的活性。在 FP 的水中检测到毒死蜱，并且电导率和 pH 值均高于 R。暴露于 FP 的蜗牛的致死率（60%）和乙酰胆碱酯酶活性降低（30%）表明，水污染导致 B. straminea 产生高毒性。
