Yu Haiming, Liu Zhihua, Chen Lingying, He Xiaoyu, Weng Yiyong, Li Weizhe, Zheng Xiaozhi, Pan Qunlong, Zhang Rongmou, Zhang Xiaoyan, Wu Wenhua
Department of Spinal Surgery, The Second Clinical Medical College of Fujian Medical University, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian 362000, China.
Key Laboratory of Chemical Materials and Green Nanotechnology, College of Chemical Engineering and Materials Science, Quanzhou Normal University, Quanzhou, Fujian 362000, China.
ACS Omega. 2024 Apr 18;9(17):19440-19450. doi: 10.1021/acsomega.4c00904. eCollection 2024 Apr 30.
Calcium silicate (CS), a new and important bioceramic bone graft material, is prepared by using eggshells, which have a porous structure and are rich in calcium ions. Furthermore, the preparation of new CS materials using eggshells and diatomaceous earth minimizes their negative impact on the environment. In this study, we prepared CS materials using a high-temperature calcination method. The composition of the material was demonstrated by X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) analysis. Scanning electron microscopy (SEM) analysis confirmed the porous structure of the CS material. We also introduced ZnO to prepare ZnO-CS with antibacterial properties and showed that ZnO-CS exhibits excellent antibacterial effects through antibacterial experiments. Subsequent mineralization experiments demonstrated that ZnO-CS promoted the formation of a hydroxyapatite layer. Furthermore, cytotoxicity experiments demonstrated that ZnO-CS had very good biosafety and promoted cell proliferation. These findings were confirmed through subsequent cell proliferation experiments. Our results indicate that the novel ZnO-CS is a promising candidate for bone tissue engineering.
硅酸钙（CS）是一种新型且重要的生物陶瓷骨移植材料，它是利用具有多孔结构且富含钙离子的蛋壳制备而成的。此外，使用蛋壳和硅藻土制备新型CS材料可将它们对环境的负面影响降至最低。在本研究中，我们采用高温煅烧法制备了CS材料。通过X射线衍射（XRD）和傅里叶变换红外光谱（FTIR）分析证明了该材料的成分。扫描电子显微镜（SEM）分析证实了CS材料的多孔结构。我们还引入了氧化锌以制备具有抗菌性能的氧化锌-CS，并通过抗菌实验表明氧化锌-CS具有优异的抗菌效果。随后的矿化实验表明，氧化锌-CS促进了羟基磷灰石层的形成。此外，细胞毒性实验表明，氧化锌-CS具有非常好的生物安全性并能促进细胞增殖。这些发现通过随后的细胞增殖实验得到了证实。我们的结果表明，新型氧化锌-CS是骨组织工程中有前景的候选材料。
